Islet formation during the neonatal development in mice.
Islet formation during the neonatal development in mice.
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DOI：
10.1371/journal.pone.0007739
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发表时间：
2009-11-06
期刊：
影响因子：
3.7
通讯作者：
Hara M
中科院分区：
文献类型：
--
作者：
Miller K;Kim A;Kilimnik G;Jo J;Moka U;Periwal V;Hara M
胰岛是胰腺外分泌部的一个独特的微器官，由分泌胰岛素的β细胞、分泌胰高血糖素的α细胞、分泌生长抑素的δ细胞、分泌胰多肽的PP细胞和分泌生长激素释放肽的ε细胞组成。胰岛还含有非内分泌细胞类型，如内皮细胞。然而，由于原位捕获这种动态事件的技术困难，对胰岛形成的机制知之甚少。我们已经开发了一种方法来监测β细胞增殖和胰岛形成的完整胰腺使用转基因小鼠中的β细胞特异性标记的荧光蛋白。内分泌细胞连续增殖，在胚胎和新生儿中形成分支索状结构。我们的研究揭示了新生儿胰腺中沿着大血管分布的长段相互连接的胰岛。α-细胞跨越细长的胰岛样结构，我们假设这代表分裂的位点，并促进离散胰岛的最终形成。我们认为胰岛的形成是通过连续的内分泌细胞增殖后的分裂过程发生的，而不是通过孤立的β细胞和胰岛的局部聚集或融合。新生儿胰岛形成的裂变过程的数学建模。
The islet of Langerhans is a unique micro-organ within the exocrine pancreas, which is composed of insulin-secreting beta-cells, glucagon-secreting alpha-cells, somatostatin-secreting delta-cells, pancreatic polypeptide-secreting PP cells and ghrelin-secreting epsilon-cells. Islets also contain non-endocrine cell types such as endothelial cells. However, the mechanism(s) of islet formation is poorly understood due to technical difficulties in capturing this dynamic event in situ. We have developed a method to monitor beta-cell proliferation and islet formation in the intact pancreas using transgenic mice in which the beta-cells are specifically tagged with a fluorescent protein. Endocrine cells proliferate contiguously, forming branched cord-like structures in both embryos and neonates. Our study has revealed long stretches of interconnected islets located along large blood vessels in the neonatal pancreas. Alpha-cells span the elongated islet-like structures, which we hypothesize represent sites of fission and facilitate the eventual formation of discrete islets. We propose that islet formation occurs by a process of fission following contiguous endocrine cell proliferation, rather than by local aggregation or fusion of isolated beta-cells and islets. Mathematical modeling of the fission process in the neonatal islet formation is also presented.
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影响因子：
9.8
作者：
Brennand K;Huangfu D;Melton D
通讯作者：
Melton D
影响因子：
7.7
作者：
BAETENS, D;STEFAN, Y;ORCI, L
通讯作者：
ORCI, L
影响因子：
2.2
作者：
Kim A;Miller K;Jo J;Kilimnik G;Wojcik P;Hara M
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Hara M
影响因子：
11.8
作者：
Teta, Monica;Rankin, Matthew M.;Kushner, Jake A.
通讯作者：
Kushner, Jake A.
DOI：
10.1152/ajpendo.00321.2002
发表时间：
2003-01-01
影响因子：
5.1
作者：
Hara, M;Wang, XY;Bell, GI
通讯作者：
Bell, GI