Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells.
Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells.
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DOI：
10.1016/j.virol.2011.01.006
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发表时间：
2011-03-30
期刊：
影响因子：
3.7
通讯作者：
Thiem SM
中科院分区：
文献类型：
--
作者：
Salem TZ;Zhang F;Xie Y;Thiem SM
加州自签名多衣壳核多角体病毒（AcMNPV）是杆状病毒中研究最多的病毒载体，也是杆状病毒表达载体系统中最常用的病毒载体。AcMNPV感染对宿主细胞的影响尚不完全清楚。利用微阵列技术研究了AcMNPV对培养的frugiperda， Sf21细胞宿主基因表达的影响。大多数宿主基因在感染的时间过程中被下调，尽管少数基因被上调。最高度上调的基因编码热休克蛋白70和一些特征不明显的蛋白。功能注释聚类鉴定出得分最高的调控基因主要包括内质网和高尔基体中蛋白质表达和运输所需的产物。在感染后大约12小时，所有的蛋白都显著下调。采用qRT-PCR验证微阵列数据。该研究首次全面概述了AcMNPV感染期间Sf21细胞的宿主转录组。
Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) is the best studied baculovirus and most commonly used virus vector for baculovirus expression vector systems. The effect of AcMNPV infection on host cells is incompletely understood. A microarray based on Spodoptera frugiperda ESTs was used to investigate the impact of AcMNPV on host gene expression in cultured S. frugiperda, Sf21 cells. Most host genes were down regulated over the time course of infection, although a small number were up-regulated. The most highly up-regulated genes encoded heat shock protein 70s and several poorly characterized proteins. Regulated genes with the highest score identified by functional annotation clustering included primarily products required for protein expression and trafficking in the ER and golgi. All were significantly down-regulated by approximately 12 hours post infection. Microarray data were validated by qRT-PCR. This study provides the first comprehensive host transcriptome overview of Sf21 cells during AcMNPV infection.
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影响因子：
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作者：
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