A Single-Organelle Optical Omics Platform for Cell Science and Biomarker Discovery.
A Single-Organelle Optical Omics Platform for Cell Science and Biomarker Discovery.
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DOI：
10.1021/acs.analchem.1c01131
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发表时间：
2021-06-15
影响因子：
7.4
通讯作者：
Prasad PN
中科院分区：
文献类型：
--
作者：
Pliss A;Kuzmin AN;Lita A;Kumar R;Celiku O;Atilla-Gokcumen GE;Gokcumen O;Chandra D;Larion M;Prasad PN
Research in fundamental cell biology and pathology could be revolutionized by developing the capacity for quantitative molecular analysis of subcellular structures. To that end, we introduce the Ramanomics platform, based on confocal Raman microspectrometry coupled to a biomolecular component analysis algorithm, which together enable to molecularly profile single organelles in a live cell environment. This emerging Omics approach categorizes the entire molecular makeup of a sample into about a dozen of general classes and subclasses of biomolecules, and quantifies their amounts in submicron volumes. A major contribution of our study is an attempt to bridge Raman spectrometry with big-data analysis in order to identify complex patterns of biomolecules in a single cellular organelle and leverage discovery of disease biomarkers. Our data reveal significant variations in organellar composition between different cell lines. We also demonstrate the merits of Ramanomics for identifying diseased cells by using prostate cancer as an example. We report large-scale molecular transformations in mitochondria, Golgi apparatus, and endoplasmic reticulum that accompany the development of prostate cancer. Based on these findings, we propose that Ramanomics datasets in distinct organelles constitute signatures of cellular metabolism in healthy and diseased states.
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DOI：
10.1016/j.bbalip.2010.04.011
发表时间：
2010-09-01
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.3390/bios7040052
发表时间：
2017-11-15
期刊：
Biosensors
影响因子：
--
作者：
Kuzmin AN;Pliss A;Prasad PN
通讯作者：
Prasad PN
影响因子：
3.7
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通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
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