Human breast cancer tissues contain abundant phosphatidylcholine(36∶1) with high stearoyl-CoA desaturase-1 expression.
Human breast cancer tissues contain abundant phosphatidylcholine(36∶1) with high stearoyl-CoA desaturase-1 expression.
复制标题
DOI：
10.1371/journal.pone.0061204
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Setou M
中科院分区：
文献类型：
--
作者：
Ide Y;Waki M;Hayasaka T;Nishio T;Morita Y;Tanaka H;Sasaki T;Koizumi K;Matsunuma R;Hosokawa Y;Ogura H;Shiiya N;Setou M
乳腺癌是全世界妇女癌症和死亡的主要原因。近年来的研究认为脂质合成与癌症进展密切相关，因为一些与脂质合成相关的酶在乳腺癌组织中过度表达。然而，脂质在乳腺癌组织中的分布尚未被研究。本研究旨在通过基质辅助激光解吸/电离成像质谱（MALDI-IMS）技术对人乳腺癌组织中的磷脂酰胆碱（PCs）和溶磷脂酰胆碱（LPCs）进行可视化，这是一种能够全面可视化分子的新技术。手术中获得29份乳腺组织样本，并进行MALDI-IMS分析。我们评估了组织中PCs和LPCs分布的异质性。含有1条单不饱和脂肪酸链和1条饱和脂肪酸链的3种PC（32∶1）、PC（34∶1）和PC(36∶1)，以及1种含有2条饱和脂肪酸链的PC（SFA-PC）相对定位于癌区，而不是其他切片（称为参照区）。此外，lpc没有显示出任何偏倚分布。癌区PC（36∶1）与PC（36∶0）、PC（36∶1）与LPC（18∶0）的相对含量均显著升高。免疫组化染色结果显示，硬脂酰辅酶a去饱和酶-1 （SCD1）蛋白表达在癌区呈积累，SCD1是MUFA的一种合成酶。考虑雌激素受体（ER）、人表皮生长因子受体2 （HER2）和Ki67的表达差异，进一步分析比值。在ER表达阳性的病变中，PC信号强度（36∶1）与PC信号强度（36∶0）之比较高。讨论了SCD1和其他酶对观察到的磷脂组成的形成的贡献。
Breast cancer is the leading cause of cancer and mortality in women worldwide. Recent studies have argued that there is a close relationship between lipid synthesis and cancer progression because some enzymes related to lipid synthesis are overexpressed in breast cancer tissues. However, lipid distribution in breast cancer tissues has not been investigated. We aimed to visualize phosphatidylcholines (PCs) and lysoPCs (LPCs) in human breast cancer tissues by performing matrix assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), which is a novel technique that enables the visualization of molecules comprehensively. Twenty-nine breast tissue samples were obtained during surgery and subjected to MALDI-IMS analysis. We evaluated the heterogeneity of the distribution of PCs and LPCs on the tissues. Three species [PC(32∶1), PC(34∶1), and PC(36∶1)] of PCs with 1 mono-unsaturated fatty acid chain and 1 saturated fatty acid chain (MUFA-PCs) and one [PC(34∶0)] of PCs with 2 saturated fatty acid chains (SFA-PC) were relatively localized in cancerous areas rather than the rest of the sections (named reference area). In addition, the LPCs did not show any biased distribution. The relative amounts of PC(36∶1) compared to PC(36∶0) and that of PC(36∶1) to LPC(18∶0) were significantly higher in the cancerous areas. The protein expression of stearoyl-CoA desaturase-1 (SCD1), which is a synthetic enzyme of MUFA, showed accumulation in the cancerous areas as observed by the results of immunohistochemical staining. The ratios were further analyzed considering the differences in expressions of the estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), and Ki67. The ratios of the signal intensity of PC(36∶1) to that of PC(36∶0) was higher in the lesions with positive ER expression. The contribution of SCD1 and other enzymes to the formation of the observed phospholipid composition is discussed.
登录
查看更多内容
影响因子：
2.4
作者：
de los Monteros, AE;Hellmén, E;de las Mulas, JM
通讯作者：
de las Mulas, JM
影响因子：
4.3
作者：
Hayasaka, Takahiro;Goto-Inoue, Naoko;Setou, Mitsutoshi
通讯作者：
Setou, Mitsutoshi
影响因子：
1.9
作者：
Chénais B;Blanckaert V
通讯作者：
Blanckaert V
影响因子：
5.7
作者：
Luyimbazi D;Akcakanat A;McAuliffe PF;Zhang L;Singh G;Gonzalez-Angulo AM;Chen H;Do KA;Zheng Y;Hung MC;Mills GB;Meric-Bernstam F
通讯作者：
Meric-Bernstam F
影响因子：
48
作者：
Cornett, Dale S.;Reyzer, Michelle L.;Caprioli, Richard M.
通讯作者：
Caprioli, Richard M.