iTRAQ-based proteomic analysis provides insights into the biological mechanism of ammonium metabolism in tea plant (Camellia sinensis L.)
iTRAQ-based proteomic analysis provides insights into the biological mechanism of ammonium metabolism in tea plant (Camellia sinensis L.)
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基于 iTRAQ 的蛋白质组学分析为茶树 (Camellia sinensis L.) 铵代谢的生物学机制提供了见解
DOI：
10.1007/s11738-020-03037-9
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发表时间：
2020-03
影响因子：
2.6
通讯作者：
Ruan Jianyun
中科院分区：
文献类型：
--
作者：
Liu Mei-Ya;Tang D;an;Zhang Qunfeng;Ruan Jianyun
茶树喜铵态氮为主要无机氮源。然而，这一偏好背后的机制尚未得到充分理解。为了更好地了解茶叶中铵的优先同化，基于iTRAQ的蛋白质组学在茶叶铵处理后0和12 h之间进行。12小时后，质量相关的成分受到积极影响，四种主要氨基酸的含量最高，初级儿茶素开始下降。儿茶素和差异表达蛋白质的变化表明，茶叶中儿茶素的含量可以影响光合作用和能量代谢所需的碳骨架的可用性。铵离子通过调控苯丙氨酸解氨酶（PAL）基因的表达和酶的活性来调控类黄酮的合成。施铵后，PAL蛋白的表达和酶活性均受到抑制，从而减少了L-苯丙氨酸转化为氨和反式肉桂酸过程中铵的释放。此外，为了加速铵的代谢，谷氨酰胺合成酶及其同工酶的表达以及谷氨酰胺合成酶的活性都得到了增强。因此，茶树在同化铵态氮的过程中，可能通过提高谷氨酰胺合成酶的活性，抑制苯丙氨酸解氨酶来平衡铵态氮的含量。这些结果为进一步研究茶树铵营养代谢提供了理论依据。
Tea plant prefers ammonium as the dominant inorganic nitrogen source. However, the mechanism behind this preference has not been fully understood. To obtain a better understanding of the preferential assimilation of ammonium in tea, iTRAQ-based proteomics was conducted between 0 and 12 h after ammonium treatment of tea leaves. The quality-related components were positively affected after 12 h, with the highest contents of the four main amino acids and with the primary catechins beginning to decrease. Changes of catechins and differentially expressed proteins suggested that contents of catechins in tea could affect the availability of carbon skeletons needed in photosynthesis and energy metabolism. The biosynthesis of flavonoid was related to ammonium through the regulation of phenylalanine ammonia-lyase (PAL) gene expression and the enzyme activity. After the application of ammonium, the expression of PAL protein and its enzyme activity were both inhibited to decrease the release of ammonium during the reaction converting l-phenylalanine to ammonia and trans-cinnamic acid. Furthermore, to accelerate the metabolism of ammonium, the expression of glutamine synthetase and its isogenes was enhanced, as well as glutamine synthetase activity. Hence, during the ammonium assimilation process, tea plants likely increase the activity of glutamine synthetase and inhibit PAL to balance the ammonium content. These results provide insights into the metabolism of ammonium nutrition orchestrated by primary and secondary metabolic pathways via glutamine synthetase and PAL in tea plant.
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发表时间：
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期刊：
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影响因子：
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