The development of a novel chemiluminescent glucose sensor using hydrophilic Co3O4@SiO2 mesoporous nanoparticles

The development of a novel chemiluminescent glucose sensor using hydrophilic Co3O4@SiO2 mesoporous nanoparticles
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使用亲水性Co3O4@SiO2介孔纳米粒子开发新型化学发光葡萄糖传感器

DOI:
10.1039/c5ay03343h
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发表时间:
2016-03
期刊:
影响因子:
3.1
通讯作者:
Zhujun Zhang
Zhujun Zhang
中科院分区:
化学3区
文献类型:
--
作者:
Xiaohua Li;Yun feng Bai;Feng Feng;Zhujun Zhang

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在此,我们提出了一种结合酶固定化技术的新型化学发光(CL)传感器。我们提出了一种通用的方法,用于制造CL传感器的基础上亲水性Co3O4@SiO2介孔纳米粒子。固定在96孔板上的Co3O4@SiO2介孔纳米粒子在碱性介质中对鲁米诺-H2 O2化学发光反应具有较高的催化活性。通过将功能化介孔二氧化硅颗粒引入海藻酸钙纤维(CAF)中来有效地包封酶,从而制备酶反应器。这种新型化学发光传感器与酶反应器相结合,具有可逆性和可重复使用性,已成功地用于高通量测定人血清中的葡萄糖。该方法是基于固定化葡萄糖氧化酶(GOD)从人血清中的葡萄糖产生H2 O2。葡萄糖浓度的线性范围为1.0 × 10−6至8.0 × 10−5 M,检测限为1.2 × 10−7 M。
Herein, we present a novel chemiluminescent (CL) sensor combined with an enzyme immobilization technique. We propose a versatile approach for the fabrication of CL sensors based on hydrophilic Co3O4@SiO2 mesoporous nanoparticles. The Co3O4@SiO2 mesoporous nanoparticles immobilized on the 96-well plates exhibited higher catalytic activity on the luminol–H2O2 CL reaction in an alkaline medium. Enzyme reactors were fabricated by incorporating functionalized mesoporous silica particles into calcium alginate fiber (CAF) to efficiently encapsulate the enzyme. The novel CL sensors combined with the enzyme reactors were reversible and reusable, which have been successfully exploited towards the high-throughput determination of glucose in human serum. The method was based on the generation of H2O2 from glucose in human serum by immobilized glucose oxidase (GOD). The linear range of the glucose concentration was 1.0 × 10−6 to 8.0 × 10−5 M and the detection limit was 1.2 × 10−7 M.
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