Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines.
Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines.
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免疫扩散沉淀素系中山羊关节炎-脑炎逆转录病毒蛋白的鉴定。
DOI:
10.1099/0022-1317-66-5-1139
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发表时间:
1985
期刊:
影响因子:
--
通讯作者:
Cheevers,WP
中科院分区:
文献类型:
--
作者:
Adams,DS;Gogolewski,RP;Barbet,AF;Cheevers,WP
Precipitin lines formed between serum from a goat infected with caprine arthritis-encephalitis virus (CAEV) and radiolabelled viral proteins in polyethylene glycolconcentrated culture medium were excised from immunodiffusion (ID) plates and analysed by polyacrylamide gel electrophoresis. The two major precipitin lines contained the 135000 mol. wt. glycoprotein (gp135) and the internal 28000 mol. wt. structural protein (p28). This method obviates the use of purified proteins or monospecific antisera to positively determine viral constituents in ID precipitin lines formed between a crude antigen preparation and antiserum against whole virus.
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