An in vivo recombinant RNA capable of autocatalytic synthesis by Qβ replicase
An in vivo recombinant RNA capable of autocatalytic synthesis by Qβ replicase
复制标题
一种能够通过 Qβ 复制酶自催化合成的体内重组 RNA
作者：
A. Munishkin;L. Voronin;A. Chetverin
在没有添加模板的情况下，长度从数十个到数百个核苷酸的各种小RNA在Qβ复制酶（Qβ噬菌体RNA依赖性RNA聚合酶）反应中自催化生长，并且在Qβ噬菌体感染的大肠杆菌细胞1-4中发现了类似的RNA。三个这样的 RNA 已被测序5-7。其中一个长度为 221 个核苷酸 (nt) 的 RNA（“MDV-1”RNA）被发现与 Qβ 噬菌体 RNA8 部分同源，这可能被认为表明其起源于 Qβ RNA 复制的副产物。为了进一步了解这些 RNA 的起源和功能，我们对一种 120 nt 长的新 RNA 进行了测序，该 RNA 是通过名义上不含 RNA 的 Qβ 复制酶制剂从自发合成的产物中分离出来的。该 RNA 的负链似乎是重组 RNA，由 Qβ RNA 的内部片段（约 80 nt 长）和大肠杆菌 tRNA1ASP 的 33 nt 长 3' 端片段组成。这似乎是细菌细胞中 RNA 重组的第一个强有力的迹象。讨论了这一发现的各种含义。
A variety of small RNAs ranging from tens to hundreds of nucieo-tides in length grow autocatalytically in a Qβ replicase (Qβ phage RNA-dependent RNA polymerase) reaction in the absence of added template, and similar RNAs are found in Qβ phage-infected Escherichia coli cellsl–4. Three such RNAs have been sequenced5–7. One of them that is 221 nucleotides (nt) long ('MDV-1' RNA) has been found to be partially homologous to Qβ phage RNA8, which might be considered as an indication of its origination from by-products of the Qβ RNA replication. To gain further insight into the origin and function of these RNAs, we have sequenced a new RNA, 120 nt long, isolated from the products of spontaneous synthesis by the nominally RNA-free Qβ replicase preparation. The minus strand of this RNA appeared to be a recombinant RNA, composed of the internal fragment of Qβ RNA (∼80 nt long) and the 33-nt-long 3′-terminal fragment of E. coli tRNA1ASP. This seems to be the first strong indication of RNA recombination in bacterial cells. The various implications of this finding are discussed.
DOI：
10.1073/pnas.80.11.3279
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MONROE, SS;SCHLESINGER, S
通讯作者：
SCHLESINGER, S
影响因子：
56.9
作者：
HOLLAND, J;SPINDLER, K;VANDEPOL, S
通讯作者：
VANDEPOL, S