Involvement of zinc in the regulation of pHi, motility, and acrosome reactions in sea urchin sperm.
Involvement of zinc in the regulation of pHi, motility, and acrosome reactions in sea urchin sperm.
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DOI：
10.1083/jcb.100.6.1817
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发表时间：
1985-06
影响因子：
7.8
通讯作者：
EPEL, D
中科院分区：
文献类型：
--
作者：
CLAPPER, DL;DAVIS, JA;LAMOTHE, PJ;PATTON, C;EPEL, D
当紫斑圆线鱼或红彩狼的精子被稀释到海水中时，就会开始运动；当接触蛋冻时，会引发顶体反应。在多种结构不同的金属螯合剂（0.1-1 mM EDTA、EGTA、菲咯啉、联吡啶、半胱氨酸或二硫苏糖醇）存在下，运动启动被延迟，顶体反应被抑制。在这两个物种的精子中检测到的金属中，极低水平的 Zn+2（0.1 µM 游离 Zn+2）独特地阻止了这种螯合剂抑制。斑鳝精子从海水中浓缩 65Zn+2，5 分钟内 EDTA 去除了积累的 65Zn+2 的 50%。由于精子活力和顶体反应在一定程度上受细胞内 pH (pHi) 调节，因此通过使用荧光 pH 敏感探针 9-氨基吖啶、EDTA 抑制两个物种的精子 pHi 来检查螯合剂对精子 pHi 的影响，而 0.1 µM 游离 Zn+2 可逆转这种 pHi 抑制。当精子被稀释到含有螯合剂的培养基中时，NH4Cl 和莫能菌素（Na+/H+ 离子载体）都会增加精子的 pHi 并逆转螯合剂对精子运动和顶体反应的抑制。这项研究的结果与微量金属（可能是锌）参与海胆精子的 pHi 调节一致，并表明了先前观察到的螯合剂对精子活力和顶体反应影响的可能机制。
When sperm of Strongylocentrotus purpuratus or Lytechinus pictus are diluted into seawater, motility is initiated; and when exposed to egg jelly, an acrosome reaction is induced. In the presence of a variety of structurally different metal chelators (0.1-1 mM EDTA, EGTA, phenanthroline, dipyridyl, cysteine, or dithiothreitol), motility initiation is delayed and the acrosome reaction is inhibited. Of the metals detected in the sperm of these two species, very low levels of Zn+2 (0.1 microM free Zn+2) uniquely prevent this chelator inhibition. L. pictus sperm concentrate 65Zn+2 from seawater, and EDTA removes 50% of the accumulated 65Zn+2 by 5 min. Since both sperm motility and acrosome reactions are in part regulated by intracellular pH (pHi), the effect of chelators on the sperm pHi was examined by using the fluorescent pH sensitive probe, 9-aminoacridine, EDTA depresses sperm pHi in both species, and 0.1 microM free Zn+2 reverses this pHi depression. When sperm are diluted into media that contain chelators, both NH4Cl and monensin (a Na+/H+ ionophore) increase the sperm pHi and reverse the chelator inhibition of sperm motility and acrosome reactions. The results of this study are consistent with the involvement of a trace metal (probably zinc) in the pHi regulation of sea urchin sperm and indicate a likely mechanism for the previously observed effects of chelators on sperm motility and acrosome reactions.
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影响因子：
3.7
作者：
MORISAWA, M;MOHRI, H
通讯作者：
MOHRI, H
影响因子：
2.7
作者：
JOHNSON, CH;CLAPPER, DL;EPEL, D
通讯作者：
EPEL, D
影响因子：
3.6
作者：
KOPF, GS;GARBERS, DL
通讯作者：
GARBERS, DL
DOI：
10.1002/mrd.1120060405
发表时间：
1982-01-01
期刊：
GAMETE RESEARCH
影响因子：
--
作者：
CLAPPER, DL;EPEL, D
通讯作者：
EPEL, D
影响因子：
--
作者：
JOHNSON, CH;EPEL, D
通讯作者：
EPEL, D