Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer.
Distinct Roles of Velvet Complex in the Development, Stress Tolerance, and Secondary Metabolism in Pestalotiopsis microspora, a Taxol Producer.
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丝绒复合物在紫杉醇生产者小孢多盘拟盘菌的发育、应激耐受性和次生代谢中的独特作用
作者：
Akhberdi O;Zhang Q;Wang D;Wang H;Hao X;Liu Y;Wei D;Zhu X
绒毡层家族蛋白已被证明在真菌次生代谢和发育中起关键作用。然而，在不同的真菌中观察到其作用存在差异。我们在此报道了对三个绒毡层复合成分VeA、VelB和LaeA在小孢拟盘多毛孢（一种先前报道的产紫杉醇真菌）中作用的观察结果。单个成员的缺失导致营养生长、孢子形成和色素沉着的延迟，表明它们在这些过程中起关键作用。突变株△velB对渗透胁迫和刚果红染料表现出超敏性，而△veA和△laeA受这些压力的影响较小，这表明只有velB是细胞壁完整性所必需的。重要的是，我们发现这些基因在小孢拟盘多毛孢次生代谢物的生物合成中发挥不同的作用。例如，先前已鉴定的聚酮化合物盘长孢内酯B的产生需要velB和laeA。相反，veA基因似乎在其生物合成中抑制盘长孢内酯B的作用。这项研究表明，绒毡层复合物的三个成分是重要的全局调控因子，但在小孢拟盘多毛孢的菌丝生长、无性繁殖和次生代谢中具有不同的作用。这项工作为进一步了解该真菌次生代谢的生物合成提供了信息。
The velvet family proteins have been shown to play critical roles in fungal secondary metabolism and development. However, variations of the roles have been observed in different fungi. We report here the observation on the role of three velvet complex components VeA, VelB, and LaeA in Pestalotiopsis microspora, a formerly reported taxol-producing fungus. Deletion of individual members led to the retardation of vegetative growth and sporulation and pigmentation, suggesting critical roles in these processes. The mutant strain △velB appeared hypersensitive to osmotic stress and the dye Congo red, whereas △veA and △laeA were little affected by the pressures, suggesting only velB was required for the integrity of the cell wall. Importantly, we found that the genes played distinct roles in the biosynthesis of secondary metabolites in P. microspora. For instance, the production of pestalotiollide B, a previously characterized polyketide, required velB and laeA. In contrast, the veA gene appeared to inhibit the pestalotiollide B (PB) role in its biosynthesis. This study suggests that the three components of the velvet complex are important global regulators, but with distinct roles in hyphal growth, asexual production, and secondary metabolism in P. microspora. This work provides information for further understanding the biosynthesis of secondary metabolism in the fungus.
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