L-3-n-butylphthalide promotes restoration after an experimental animal model of intracerebral hemorrhage.
L-3-n-butylphthalide promotes restoration after an experimental animal model of intracerebral hemorrhage.
复制标题
L-3-正丁基苯酞促进实验动物脑出血模型后的恢复
DOI：
10.7150/ijms.60342
复制
发表时间：
2021
影响因子：
3.6
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Pi Z;Liu J;Xiao H;Hu Z
脑出血是一种具有高发病率和死亡率的破坏性脑卒中类型，脑出血的有效治疗方法仍有待探索。l -3-正丁苯酞（NBP）广泛应用于缺血性脑卒中的治疗。然而，很少有研究评估NBP对脑出血的治疗效果。因此，本研究旨在探讨NBP对脑出血的影响及其可能的机制。将大鼠随机分为假手术组、脑出血+生理盐水组、脑出血+ NBP组。建立胶原酶诱导SD大鼠脑出血模型。改良的加西亚JH评分用于检测大鼠的神经功能缺陷。采用Western Blot和免疫组织化学方法检测血肿周围UBIAD1和caspase-3的表达水平。TUNEL染色检测细胞凋亡率。结果表明，NBP上调UBIAD1的表达，减少血肿周围区凋亡细胞，改善神经功能缺损。综上所述，我们的研究为NBP在脑出血治疗中的应用提供了一些新的证据。
Intracerebral hemorrhage (ICH) is a devastating type of stroke with high morbidity and mortality, and the effective therapies for ICH remain to be explored. L-3-n-butylphthalide (NBP) is widely used in the treatment of ischemic stroke. However, few studies evaluated the therapeutic effects of NBP on ICH. Therefore, the present study aims to evaluate the effects of NBP on ICH and its potential mechanism. The rats were randomly divided into sham-operated group, saline-treated (ICH + saline) group, and NBP-treated (ICH + NBP) group. The ICH model of SD rats induced by IV collagenase was established. The modified Garcia JH score was used to detect the neurological deficit in rats. Western Blot and immunohistochemistry analysis was applied to test the levels of UBIAD1 and caspase-3 expressions in the perihematomal region. The rates of apoptotic cells were detected by TUNEL staining. The results showed that NBP up-regulated the expression of UBIAD1, reduced the apoptotic cells in the perihematomal region, and improved the neurological deficit. Taken together, our study added some new evidence to the application of NBP in ICH treatment.
登录
查看更多内容
影响因子：
3.7
作者：
Orr A;Dubé MP;Marcadier J;Jiang H;Federico A;George S;Seamone C;Andrews D;Dubord P;Holland S;Provost S;Mongrain V;Evans S;Higgins B;Bowman S;Guernsey D;Samuels M
通讯作者：
Samuels M
影响因子：
64.8
作者：
Nakagawa, Kimie;Hirota, Yoshihisa;Okano, Toshio
通讯作者：
Okano, Toshio
影响因子：
8.3
作者：
GARCIA, JH;WAGNER, S;HU, XJ
通讯作者：
HU, XJ
影响因子：
48
作者：
van Asch, Charlotte J. J.;Luitse, Merel J. A.;Klijn, Catharina J. M.
通讯作者：
Klijn, Catharina J. M.
影响因子：
5.6
作者：
Niu, Xiao-Li;Jiang, Xin;Lv, Pei-Yuan
通讯作者：
Lv, Pei-Yuan