Effect of BLV Infection on the Immune Function of Polymorphonuclear Neutrophil in Dairy Cows.
Effect of BLV Infection on the Immune Function of Polymorphonuclear Neutrophil in Dairy Cows.
复制标题
BLV感染对奶牛中多形核中性粒细胞免疫功能的影响。
DOI：
10.3389/fvets.2021.737608
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发表时间：
2021
影响因子：
3.2
通讯作者：
Wu R
中科院分区：
文献类型：
--
作者：
Lv G;Wang H;Wang J;Lian S;Wu R
地方性牛白血病是由牛白血病病毒（BLV）引起的迟发性肿瘤感染。BLV感染会阻碍免疫系统的功能并诱发其他疾病，从而对受感染奶牛的生产性能和健康产生负面影响。中性粒细胞（PMN）作为抵御外来病原微生物入侵的第一道防线，在奶牛免疫系统中起着至关重要的作用。但关于BLV感染对奶牛中性粒细胞免疫功能影响的研究较少。因此，本试验旨在通过检测BLV感染对不同BLV前病毒载量奶牛中性粒细胞（PMN）的趋化、迁移、粘附、吞噬、呼吸爆发功能和NETs形成的影响，探讨BLV感染对PMN免疫功能的影响及其作用机制。实验结果表明，BLV感染对PMN的吞噬功能无明显影响，但对PMN的迁移和呼吸爆发功能有抑制作用，这种影响与BLV前病毒负荷密切相关。在高BLV原病毒负荷下，PMN产生大量的NETs、趋化因子CXCL 7、粘附分子CD 18以及促炎因子IL-8和TNF-α，引发炎症反应和组织损伤。本研究结果有助于揭示BLV感染导致奶牛乳腺炎高发的原因。
Enzootic bovine leukemia is a late-onset, neoplastic infection caused by the bovine leukemia virus (BLV). BLV infection hinders the function of the immune system and induces other diseases, which negatively affects the performance and health of the infected cows. As the first line of defense against invading foreign pathogenic microorganisms, polymorphonuclear neutrophil (PMN) plays a vital role in the immune system of dairy cows. However, research on the effect of BLV infection on the immune function of PMN in dairy cows is scarce. Therefore, this experiment aimed to elucidate the effects and effect mechanisms of BLV infection on the immune function of PMN in dairy cows with different BLV provirus loads by detecting the chemotaxis, migration, adhesion, phagocytosis, respiratory burst function, and the formation of NETs. The experimental results showed that BLV infection had no significant effect on the phagocytosis of PMN but inhibited their migration and respiratory burst function, and the effects were closely related to the BLV provirus load. Under high BLV provirus load, PMN produced large amounts of NETs, chemokine CXCL7, adhesion molecule CD18, and pro-inflammatory factors IL-8 and TNF-α, triggering inflammatory responses, and tissue damage. The results of this study will help reveal the reason why BLV infection causes the high incidence of mammary gland inflammation in dairy cows.
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DOI：
10.1292/jvms.14-0624
发表时间：
2015-09
期刊：
The Journal of veterinary medical science
影响因子：
--
作者：
Mekata H;Sekiguchi S;Konnai S;Kirino Y;Horii Y;Norimine J
通讯作者：
Norimine J
影响因子：
1
作者：
Juliarena, Marcela A.;Gutierrez, Silvina E.;Ceriani, Carolina
通讯作者：
Ceriani, Carolina
影响因子：
3.5
作者：
Juliarena, Marcela A.;Barrios, Clarisa N.;Esteban, Eduardo N.
通讯作者：
Esteban, Eduardo N.
影响因子：
4.4
作者：
Ikebuchi R;Konnai S;Okagawa T;Yokoyama K;Nakajima C;Suzuki Y;Murata S;Ohashi K
通讯作者：
Ohashi K
影响因子：
3.5
作者：
Norby, B.;Bartlett, P. C.;Erskine, R. J.
通讯作者：
Erskine, R. J.