Variation in the chemical composition of wheat straw: the role of tissue ratio and composition.

Variation in the chemical composition of wheat straw: the role of tissue ratio and composition.
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DOI:
10.1186/s13068-014-0121-y
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发表时间:
2014
影响因子:
6.3
通讯作者:
Waldron KW
Waldron KW
中科院分区:
工程技术1区
文献类型:
--
作者:
Collins SR;Wellner N;Martinez Bordonado I;Harper AL;Miller CN;Bancroft I;Waldron KW

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小麦秸秆是第二代乙醇生产的有吸引力的基质,因为它将补充和增加小麦生产,而不是与粮食生产竞争。然而,与木质纤维素生物质的其他来源一样,即使来自单一物种,由于不同的组织和细胞类型,它在性质上是异质的,这对糖化效率有影响。本研究的目的是使用傅里叶变换红外(FTIR)光谱和偏最小二乘(PLS)建模,以快速筛选小麦品种的组成组织的水平,碳水化合物的组成和木质素含量,以及简单的交联酚类物质,如阿魏酸和二阿魏酸的水平。采用傅立叶变换红外光谱技术和偏最小二乘法(PLS)建模方法对小麦秸秆生物质的组织和化学组成进行了分析。开发预测模型以评估细胞壁糖、细胞壁酚类物质和酸不溶性木质素的浓度的可变性。模型的主要糖,酚类物质和木质素进行了验证,然后用来评估在总生物量组成的变化在90个品种的小麦生长超过两个赛季。虽然碳水化合物和木质素组分在品种之间存在差异,但这主要反映了组分组织比例的差异,而不是这些组织组成的差异。进一步的分析表明,以摩尔%为基础,组织内糖的相对水平变化很小。简单的酚类物质和组织之间没有明显的联系。研究结果为通过选择具有合适组织比的品种来提高生物质质量以生产生物燃料提供了依据。本文的在线版本(doi:10.1186/s13068-014-0121-y)包含补充材料,可供授权用户使用。
Wheat straw is an attractive substrate for second generation ethanol production because it will complement and augment wheat production rather than competing with food production. However, like other sources of lignocellulosic biomass, even from a single species, it is heterogeneous in nature due to the different tissues and cell types, and this has implications for saccharification efficiency. The aim of this study has been to use Fourier transform infrared (FTIR) spectroscopy and Partial least squares (PLS) modelling to rapidly screen wheat cultivars for the levels of component tissues, the carbohydrate composition and lignin content, and the levels of simple cross-linking phenolics such as ferulic and diferulic acids. FTIR spectroscopy and PLS modelling was used to analyze the tissue and chemical composition of wheat straw biomass. Predictive models were developed to evaluate the variability in the concentrations of the cell wall sugars, cell wall phenolics and acid-insoluble lignin. Models for the main sugars, phenolics and lignin were validated and then used to evaluate the variation in total biomass composition across 90 cultivars of wheat grown over two seasons. Whilst carbohydrate and lignin components varied across the varieties, this mainly reflected differences in the ratios of the component tissues rather than differences in the composition of those tissues. Further analysis indicated that on a mol% basis, relative levels of sugars within the tissues varied to only a small degree. There were no clear associations between simple phenolics and tissues. The results provide a basis for improving biomass quality for biofuels production through selection of cultivars with appropriate tissue ratios. The online version of this article (doi:10.1186/s13068-014-0121-y) contains supplementary material, which is available to authorized users.
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