Dietary fat influences the expression of contractile and metabolic genes in rat skeletal muscle.
Dietary fat influences the expression of contractile and metabolic genes in rat skeletal muscle.
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DOI：
10.1371/journal.pone.0080152
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ikeuchi Y
中科院分区：
文献类型：
--
作者：
Mizunoya W;Iwamoto Y;Shirouchi B;Sato M;Komiya Y;Razin FR;Tatsumi R;Sato Y;Nakamura M;Ikeuchi Y
膳食脂肪在肥胖、脂肪代谢和心血管疾病中起着重要作用。为了确定不同类型饮食脂肪的摄入是否影响支配骨骼肌代谢和收缩特性的肌肉纤维类型，我们用来自不同脂肪来源的15%脂肪饮食喂养雄性Wistar大鼠。分别以大豆油(富含n-6多不饱和脂肪酸)、鱼油(富含n-3多不饱和脂肪酸)或猪油(低多不饱和脂肪酸)组成饲料喂养大鼠4周。用肌球蛋白重链(MyHC)亚型作为生物标志物来描述骨骼肌纤维类型。与豆油摄入量相比，鱼油摄入量显著降低了快速型优势肌肉指长伸肌中快速型MyHC2B的含量，而增加了中间型MyHC2X的含量。同时，鱼油喂养大鼠的MyHC2X基因表达水平显著高于豆油喂养大鼠。饲喂猪油大鼠的MyHC亚型组成介于鱼油和豆油饲喂大鼠之间。与饲喂大豆油和猪油的大鼠相比，饲喂鱼油的大鼠EDL中线粒体解偶联蛋白3、丙酮酸脱氢酶激酶4和孔蛋白mRNA的水平显著上调，这意味着氧化代谢的激活。相比之下，在比目鱼肌中没有观察到MyHC亚型的组成变化，比目鱼肌是一种慢型优势肌。血清和肌肉中的脂肪酸组成明显受饮食脂肪摄入类型的影响。综上所述，膳食脂肪影响了快速型优势骨骼肌中与收缩和代谢特性相关的基因的表达，摄取鱼油后氧化代谢的激活比豆油摄取后更为明显。
Dietary fat plays a major role in obesity, lipid metabolism, and cardiovascular diseases. To determine whether the intake of different types of dietary fats affect the muscle fiber types that govern the metabolic and contractile properties of the skeletal muscle, we fed male Wistar rats with a 15% fat diet derived from different fat sources. Diets composed of soybean oil (n-6 polyunsaturated fatty acids (PUFA)-rich), fish oil (n-3 PUFA-rich), or lard (low in PUFAs) were administered to the rats for 4 weeks. Myosin heavy chain (MyHC) isoforms were used as biomarkers to delineate the skeletal muscle fiber types. Compared with soybean oil intake, fish oil intake showed significantly lower levels of the fast-type MyHC2B and higher levels of the intermediate-type MyHC2X composition in the extensor digitorum longus (EDL) muscle, which is a fast-type dominant muscle. Concomitantly, MyHC2X mRNA levels in fish oil-fed rats were significantly higher than those observed in the soybean oil-fed rats. The MyHC isoform composition in the lard-fed rats was an intermediate between that of the fish oil and soybean oil-fed rats. Mitochondrial uncoupling protein 3, pyruvate dehydrogenase kinase 4, and porin mRNA showed significantly upregulated levels in the EDL of fish oil-fed rats compared to those observed in soybean oil-fed and lard-fed rats, implying an activation of oxidative metabolism. In contrast, no changes in the composition of MyHC isoforms was observed in the soleus muscle, which is a slow-type dominant muscle. Fatty acid composition in the serum and the muscle was significantly influenced by the type of dietary fat consumed. In conclusion, dietary fat affects the expression of genes related to the contractile and metabolic properties in the fast-type dominant skeletal muscle, where the activation of oxidative metabolism is more pronounced after fish oil intake than that after soybean oil intake.
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DOI：
10.1016/j.bbapap.2009.11.023
发表时间：
2010-08
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Dominy JE Jr;Lee Y;Gerhart-Hines Z;Puigserver P
通讯作者：
Puigserver P
影响因子：
64.5
作者：
Narkar VA;Downes M;Yu RT;Embler E;Wang YX;Banayo E;Mihaylova MM;Nelson MC;Zou Y;Juguilon H;Kang H;Shaw RJ;Evans RM
通讯作者：
Evans RM
影响因子：
5.9
作者：
Lee, Mak-Soon;Kim, In-Hwan;Kim, Yangha
通讯作者：
Kim, Yangha
影响因子：
2.2
作者：
Patterson E;Wall R;Fitzgerald GF;Ross RP;Stanton C
通讯作者：
Stanton C
影响因子：
15.9
作者：
Kitamura, Tadahiro;Kitamura, Yukari Ido;Accili, Domenico
通讯作者：
Accili, Domenico