Significance, specificity, and ultrastructural localization of HMB-45 antigen in pigmented ocular tumors.
Significance, specificity, and ultrastructural localization of HMB-45 antigen in pigmented ocular tumors.
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HMB-45 抗原在眼部色素肿瘤中的意义、特异性和超微结构定位。
DOI：
10.1016/s0161-6420(93)31668-4
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发表时间：
1993
期刊：
影响因子：
13.7
通讯作者：
Hans
中科院分区：
文献类型：
--
作者：
K. Steuhl;J. Rohrbach;Marcus Knorr;Hans
目的：评价单克隆HMB-45抗体在黑素细胞和非黑素细胞眼部肿瘤中的表达，寻找“活化”的细胞亚群，试图区分良性和恶性黑素细胞，比较HMB-45和S100的活性，并确定该肿瘤标志物对黑素细胞眼部病变的特异性。方法：对10例获得性结膜黑素瘤、19例结膜痣、34例结膜黑素瘤、69例葡萄膜黑素瘤、20例眼睑基底细胞癌、20例囊性皮样瘤、15例眼睑血管瘤、20例结膜乳头状瘤、20例鳞状细胞癌、20例胬肉、11例皮脂腺癌、10例视网膜母细胞瘤、5例脉络膜转移癌进行免疫组织学检查。采用亲和素-生物素过氧化物酶技术和单克隆HMB-45抗体。研究了S100蛋白在黑色素细胞肿瘤中的分布，并进行了比较。为了定位HMB-45抗原，应用免疫电镜对葡萄膜黑色素瘤的低辛基包埋组织进行了研究。结果：95%以上的结膜和脉络膜黑色素瘤表达HMB-45抗原，而所有结膜黑色素瘤和91%的葡萄膜黑色素瘤均表达S100抗原。在结膜的良性黑色素细胞病变（痣和黑素细胞）中，特别是上皮内和结膜成分被HMB-45染色，在肿瘤侵袭部位，浸润细胞显示HMB-45反应性增加。总体而言，HMB-45抗原在黑素细胞瘤中的分布不均匀，而S100抗原在黑素细胞瘤中的分布不均匀。所有非黑素细胞性眼部肿瘤均未发现HMB-45表达。视网膜色素上皮和无瘤脉络膜HMB-45阴性。HMB-45抗原在II期和III期的黑素体中被免疫电镜发现。结论：HMB-45免疫组织学有助于区分黑素细胞性和非黑素细胞性眼部肿瘤，并经常明确肿瘤侵袭的前沿。较强的HMB-45反应性可能反映了黑素细胞的活化，但良性和恶性黑素细胞之间并没有明确的界限。
Purpose:To evaluate the expression of the monoclonal HMB-45 antibody in melanocytic and nonmelanocytic ocular tumors and seek "activated" cellular subpopulations in an attempt to distinguish between benign and malignant melanocytes, to compare HMB-45 and S100 activity, and to determine the specificity of this tumor marker for melanocytic ocular lesions.Methods:Immunohistologic investigations were performed with paraffin-embedded tissue of 10 acquired conjunctival melanoses,19 conjunctival nevi, 34 conjunctival melanomas, 69 uveal melanomas, 20 basal cell carcinomas of the lid, 20 cystic dermoids, 15 hemangiomas of the lid, 20 conjunctival papillomas, 20 squamous cell carcinomas, 20 pterygia,11 sebaceous gland carcinomas, 10 retinoblastomas, and 5 choroidal metastatic carcinomas. The avidin-biotin peroxidase technique and monoclonal HMB-45 antibody were used. The distribution of S100 protein was studied in the melanocytic tumors for comparison. To localize the HMB-45 antigen, lowicryl-embedded tissue of uveal melanomas was investigated,, immunoelectron microscopically.Results:More than 95% of the conjunctival and choroidal melanomas expressed the HMB-45 antigen, while S100 was found in all melanomas of the conjunctiva and in 91 % of the uveal melanomas. In benign melanocytic lesions of the conjunctiva (nevi and melanocytes), especially the intraepithelial and junctional components stained with HMB-45, and at the site of tumor invasion, infiltrating cells showed increased HMB-45 reactivity. On the whole, HMB-45 antigen was less evenly distributed in the melanocytic tumors investigated than S100 antigen. All nonmelanocytic ocular tumors revealed no HMB-45 expression. Retinal pigment epithelium and tumor-free choroid were negative for HMB-45. The HMB-45 antigen was immunoelectron microscopically found in melanosomes at stages II and III.Conclusion:HMB-45 immunohistology helps in distinguishing melanocytic from nonmelanocytic ocular tumors and often clarifies the front of tumor invasion. The stronger HMB-45 reactivity probably reflects melanocytic activation, but a sharp line between benign and malignant melanocytes cannot be drawn.
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DOI：
10.1001/archopht.1992.01080150097036
发表时间：
1992
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Wang,MX;EarleyJr,JJ;Shields,JA;Donoso,LA
通讯作者：
Donoso,LA
结膜黑变病和黑色素瘤。
影响因子：
13.7
作者：
Folberg,R;McLean,IW;Zimmerman,LE
通讯作者：
Zimmerman,LE
影响因子：
4.2
作者：
MCLEAN, IW;FOSTER, WD;GAMEL, JW
通讯作者：
GAMEL, JW
结膜黑素细胞病变的 HMB-45 免疫组织化学染色。
影响因子：
13.7
作者：
McDonnell,JM;Sun,YY;Wagner,D
通讯作者：
Wagner,D