Unbiased whole-genome deep sequencing of human and porcine stool samples reveals circulation of multiple groups of rotaviruses and a putative zoonotic infection.
Unbiased whole-genome deep sequencing of human and porcine stool samples reveals circulation of multiple groups of rotaviruses and a putative zoonotic infection.
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DOI：
10.1093/ve/vew027
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发表时间：
2016-07
期刊：
影响因子：
5.3
通讯作者：
VIZIONS Consortium
中科院分区：
文献类型：
--
作者：
Phan MVT;Anh PH;Cuong NV;Munnink BBO;van der Hoek L;My PT;Tri TN;Bryant JE;Baker S;Thwaites G;Woolhouse M;Kellam P;Rabaa MA;Cotten M;VIZIONS Consortium
对人类临床病例和动物宿主中的人畜共患病病毒进行协调和同步监测，为识别种间病毒移动提供了机会。轮状病毒（RV）是引起人类和动物病毒性胃肠炎的重要病原。在这项研究中，我们记录了RV的多样性内共处的人类和动物样本从湄公河三角洲地区的越南使用引物独立的，不可知的，深度测序方法。共对296份粪便样本（146份来自人类腹泻患者，150份来自生活在同一地理区域的猪）进行了直接测序，生成了60份人类轮状病毒（均为A组）和31份猪轮状病毒（13份A组，7份B组，6份C组和5份H组）的基因组序列。系统发育分析显示多种不同的RV A组（RVA）基因型/毒株的共循环，其中许多与许可的RVA疫苗的毒株组分不同，以及猪中相当大的病毒多样性，包括B、C和H组轮状病毒的全基因组，这些病毒在越南之前均未报告。此外，在同一地区的人类患者和猪中检测到非典型RVA基因型星座（G4-P[6]-I1-R1-C1-M1-A8-N1-T7-E1-H1），为人畜共患病事件提供了一些证据。
Coordinated and synchronous surveillance for zoonotic viruses in both human clinical cases and animal reservoirs provides an opportunity to identify interspecies virus movement. Rotavirus (RV) is an important cause of viral gastroenteritis in humans and animals. In this study, we document the RV diversity within co-located humans and animals sampled from the Mekong delta region of Vietnam using a primer-independent, agnostic, deep sequencing approach. A total of 296 stool samples (146 from diarrhoeal human patients and 150 from pigs living in the same geographical region) were directly sequenced, generating the genomic sequences of sixty human rotaviruses (all group A) and thirty-one porcine rotaviruses (thirteen group A, seven group B, six group C, and five group H). Phylogenetic analyses showed the co-circulation of multiple distinct RV group A (RVA) genotypes/strains, many of which were divergent from the strain components of licensed RVA vaccines, as well as considerable virus diversity in pigs including full genomes of rotaviruses in groups B, C, and H, none of which have been previously reported in Vietnam. Furthermore, the detection of an atypical RVA genotype constellation (G4-P[6]-I1-R1-C1-M1-A8-N1-T7-E1-H1) in a human patient and a pig from the same region provides some evidence for a zoonotic event.
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影响因子：
4.5
作者：
Alam MM;Pun SB;Gauchan P;Yokoo M;Doan YH;Tran TN;Nakagomi T;Nakagomi O;Pandey BD
通讯作者：
Pandey BD
影响因子：
168.9
作者：
Armah, George E.;Sow, Samba O.;Neuzil, Kathleen M.
通讯作者：
Neuzil, Kathleen M.
影响因子：
3.6
作者：
Anh, DD;Thiem, VD;Glass, RI
通讯作者：
Glass, RI
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
9.4
作者：
BOOM, R;SOL, CJA;VANDERNOORDAA, J
通讯作者：
VANDERNOORDAA, J