Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD.
Progenitors of skeletal muscle satellite cells express the muscle determination gene, MyoD.
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DOI：
10.1016/j.ydbio.2009.05.554
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发表时间：
2009-08-01
影响因子：
2.7
通讯作者：
Goldhamer, David J.
中科院分区：
文献类型：
--
作者：
Kanisicak, Onur;Mendez, Julio J.;Yamamoto, Shoko;Yamamoto, Masakazu;Goldhamer, David J.
关键词：
卫星细胞是负责骨骼肌生长和再生的组织特异性干细胞。尽管卫星细胞在近 50 年前就已被发现，但其来源的祖细胞群的身份仍然存在争议。我们开发了 MyoDiCre 敲入小鼠，并使用 Cre/lox 谱系分析来确定卫星细胞祖细胞是否表达 MyoD（肌源性定型的标记物）。分别使用 R26R-EYFP 和 R26R (β-gal) 报告小鼠，通过分离的肌纤维的共聚焦显微镜和分离后立即固定的肌肉组织的电子显微镜观察来确定卫星细胞的重组状态。我们发现，基本上所有与肢体和体壁肌肉组织以及膈肌和眼外肌相关的成体卫星细胞都起源于 MyoD+ 祖细胞。新生儿卫星细胞进行了 Cre 重组，但只有一小部分表现出持续的 Cre 表达，表明大多数卫星细胞在产前就表达了 MyoD。我们还表明，MyoD 缺失小鼠中卫星细胞的发育并不是由于 MyoD 非表达谱系的功能补偿。结果表明，卫星细胞源自定型肌源祖细胞，无论相关肌肉组织的解剖位置、胚胎起源或生理特性如何。
Satellite cells are tissue-specific stem cells responsible for skeletal muscle growth and regeneration. Although satellite cells were identified almost 50 years ago, the identity of progenitor populations from which they derive remains controversial. We developed MyoDiCre knockin mice, and used Cre/lox lineage analysis to determine whether satellite cell progenitors express MyoD, a marker of myogenic commitment. Recombination status of satellite cells was determined by confocal microscopy of isolated muscle fibers and by electron microscopic observation of muscle tissue fixed immediately following isolation, using R26R-EYFP and R26R (β-gal) reporter mice, respectively. We show that essentially all adult satellite cells associated with limb and body wall musculature, as well as the diaphragm and extraocular muscles, originate from MyoD+ progenitors. Neonatal satellite cells were Cre-recombined, but only a small minority exhibited ongoing Cre expression, indicating that most satellite cells had expressed MyoD prenatally. We also show that satellite cell development in MyoD-null mice is not due to functional compensation by MyoD non-expressing lineages. The results suggest that satellite cells are derived from committed myogenic progenitors, irrespective of the anatomical location, embryological origin, or physiological properties of associated musculature.
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影响因子：
10.5
作者：
Kassar-Duchossoy, L;Giacone, E;Tajbakhsh, S
通讯作者：
Tajbakhsh, S
影响因子：
2.7
作者：
Cornelison, DDW;Olwin, BB;Wold, BJ
通讯作者：
Wold, BJ
影响因子：
4.6
作者：
Gensch, Nicole;Borchardt, Thilo;Braun, Thomas
通讯作者：
Braun, Thomas
影响因子：
2.7
作者：
Cornelison, DDW;Wold, BJ
通讯作者：
Wold, BJ
影响因子：
64.8
作者：
Kassar-Duchossoy, L;Gayraud-Morel, B;Tajbakhsh, S
通讯作者：
Tajbakhsh, S