Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin.
Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin.
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DOI：
10.1038/s41598-023-38036-6
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发表时间：
2023-07-13
影响因子：
4.6
通讯作者：
Harding, Stephen E. E.
中科院分区：
文献类型：
--
作者：
Chun, Taewoo;Pattem, Jacob;Gillis, Richard B. B.;Dinu, Vlad T. T.;Yakubov, Gleb E. E.;Corfield, Anthony P. P.;Harding, Stephen E. E.
糖肽类抗生素常用于眼科治疗革兰氏阳性菌感染。然而，抗生素与粘蛋白的聚集性相互作用可能导致长期接触并增加耐药物种的风险。本研究重点评价最后一道防线糖肽类抗生素替考拉宁与眼粘蛋白模型之间的潜在相互作用，采用精密无基质流体动力学和显微镜技术：分析型超离心沉降速度（SV-AUC）、动态光散射（DLS）和原子力显微镜（AFM）。对于较高剂量（1.25 mg/mL和12.5 mg/mL）的替考拉宁混合物，在沉降系数（SV-AUC）和流体动力学半径（DLS）的分布中均显示与牛颌下粘蛋白（BSM）相互作用并聚集。对于较高浓度，通过AFM证实聚集体的存在。我们建议替考拉宁滴眼液制剂的给药浓度应< 1.25 mg/mL，以避免潜在的有害聚集。
Glycopeptide antibiotics are regularly used in ophthalmology to treat infections of Gram-positive bacteria. Aggregative interactions of antibiotics with mucins however can lead to long exposure and increases the risk of resistant species. This study focuses on the evaluation of potential interactions of the last line of defence glycopeptide antibiotic teicoplanin with an ocular mucin model using precision matrix free hydrodynamic and microscopic techniques: sedimentation velocity in the analytical ultracentrifuge (SV-AUC), dynamic light scattering (DLS) and atomic force microscopy (AFM). For the mixtures of teicoplanin at higher doses (1.25 mg/mL and 12.5 mg/mL), it was shown to interact and aggregate with bovine submaxillary mucin (BSM) in the distributions of both sedimentation coefficients by SV-AUC and hydrodynamic radii by DLS. The presence of aggregates was confirmed by AFM for higher concentrations. We suggest that teicoplanin eye drop formulations should be delivered at concentrations of < 1.25 mg/mL to avoid potentially harmful aggregations.
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通过分子流体动力学研究的水溶液中糖肽抗生素二十二糖蛋白A2的自我关联。
影响因子：
4.6
作者：
通讯作者：
--
影响因子：
3
作者：
Dinu, Vlad;Gillis, Richard B.;Fisk, Ian D.
通讯作者：
Fisk, Ian D.
影响因子：
4.1
作者：
Deacon, MP;McGurk, S;Harding, SE
通讯作者：
Harding, SE
影响因子：
5.3
作者：
Blaskovich MAT;Hansford KA;Butler MS;Jia Z;Mark AE;Cooper MA
通讯作者：
Cooper MA
影响因子：
4.1
作者：
HARDING, SE;JOHNSON, P
通讯作者：
JOHNSON, P