Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics.
Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics.
复制标题
通过分子流体动力学研究的水溶液中糖肽抗生素二十二糖蛋白A2的自我关联。
DOI：
10.1038/s41598-023-28740-8
复制
发表时间：
2023-02-03
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
天然糖肽类抗生素替考拉宁用于治疗严重的革兰氏阳性相关细菌感染，可静脉、肌内、局部（眼部感染）或口服给药。它也被认为是针对SARS-CoV-2病毒感染。在分析超离心法和毛细管（滚球）粘度法中，采用沉降速度和沉降平衡，在氯化磷酸盐缓冲液（pH 6.8，I = 0.10 M）中检查替考拉宁A2（M1 = 1880 g/mol）的流体动力学性质。在浓度范围内，0-10 mg/mL替考拉宁A2的自缔合平台> 1 mg/mL，摩尔质量为（35，400 ± 1000）g/mol，相当于~（19 ± 1）个聚体，沉降系数s20，w = ~ 4.65 S。发现特性粘度[]为（3.2 ± 0.1）mL/g：这两者、s20，w值和动态光散射的流体动力学半径均与球状大分子组装体一致，通过动态水合过程的溶胀比约为2。
The natural glycopeptide antibiotic teicoplanin is used for the treatment of serious Gram-positive related bacterial infections and can be administered intravenously, intramuscularly, topically (ocular infections), or orally. It has also been considered for targeting viral infection by SARS-CoV-2. The hydrodynamic properties of teicoplanin A2 (M1 = 1880 g/mol) were examined in phosphate chloride buffer (pH 6.8, I = 0.10 M) using sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge together with capillary (rolling ball) viscometry. In the concentration range, 0–10 mg/mL teicoplanin A2 was found to self-associate plateauing > 1 mg/mL to give a molar mass of (35,400 ± 1000) g/mol corresponding to ~ (19 ± 1) mers, with a sedimentation coefficient s20, w = ~ 4.65 S. The intrinsic viscosity [] was found to be (3.2 ± 0.1) mL/g: both this, the value for s20,w and the hydrodynamic radius from dynamic light scattering are consistent with a globular macromolecular assembly, with a swelling ratio through dynamic hydration processes of ~ 2.
登录
查看更多内容
影响因子：
12.7
作者：
Ceccarelli, Giancarlo;Alessandri, Francesco;Venditti, Mario
通讯作者：
Venditti, Mario
DOI：
10.1007/s00249-018-1321-3
发表时间：
2018-10
期刊：
European biophysics journal : EBJ
影响因子：
--
作者：
Harding SE
通讯作者：
Harding SE
影响因子：
5.3
作者：
Blaskovich MAT;Hansford KA;Butler MS;Jia Z;Mark AE;Cooper MA
通讯作者：
Cooper MA
DOI：
10.1016/j.bbagen.2017.05.011
发表时间：
2017-08
期刊：
Biochimica et biophysica acta. General subjects
影响因子：
--
作者：
Hughes CS;Longo E;Phillips-Jones MK;Hussain R
通讯作者：
Hussain R
影响因子：
4.1
作者：
HARDING, SE;JOHNSON, P
通讯作者：
JOHNSON, P