Normal table of postembryonic zebrafish development: staging by externally visible anatomy of the living fish.

Normal table of postembryonic zebrafish development: staging by externally visible anatomy of the living fish.
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DOI:
10.1002/dvdy.22113
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发表时间:
2009-12
期刊:
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子:
--
通讯作者:
Engeszer RE
Engeszer RE
中科院分区:
其他
文献类型:
--
作者:
Parichy DM;Elizondo MR;Mills MG;Gordon TN;Engeszer RE

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The zebrafish is a premier model organism yet lacks a system for assigning post-embryonic fish to developmental stages. To provide such a staging series, we describe post-embryonic changes in several traits that are visible under brightfield illumination or through vital staining and epiflourescent illumination. These include the swim bladder, median and pelvic fins, pigment pattern, scale formation, larval fin fold and skeleton. We further identify milestones for placing post-embryonic fish into discrete stages. We relate these milestones to changes in size and age and show that size is a better indicator of developmental progress than is age. We also examine how relationships between size and developmental progress vary with temperature and density, and we document the effects of histological processing on size. To facilitate post-embryonic staging, we provide images of reference individuals that have attained specific developmental milestones and are of defined sizes. Finally we provide guidelines for reporting stages that provide information on both discrete and continuous changes in growth and development.
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