Maltose-Functionalized Hydrophilic Magnetic Nanoparticles with Polymer Brushes for Highly Selective Enrichment of N-Linked Glycopeptides.
Maltose-Functionalized Hydrophilic Magnetic Nanoparticles with Polymer Brushes for Highly Selective Enrichment of N-Linked Glycopeptides.
复制标题
带有聚合物刷的麦芽糖功能化亲水性磁性纳米粒子用于高选择性富集 N-连接糖肽
DOI：
10.1021/acsomega.7b01788
复制
发表时间：
2018-02-28
期刊：
影响因子：
4.1
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Bi C;Liang Y;Shen L;Tian S;Zhang K;Li Y;He X;Chen L;Zhang Y
从复杂的生物溶液中高效富集糖蛋白/糖肽在生物医学科学，特别是生物标志物研究中具有重要意义。在本工作中，通过简单的两步修饰，设计并合成了高亲水性的聚乙烯亚胺共轭聚麦芽糖聚合物刷子功能化磁性Fe3O4纳米粒子(简称Fe3O4-PEI-p麦芽糖)。得到的超亲水Fe3O4-PEI-pMaltose纳米颗粒具有高选择性(HRP和牛血清白蛋白(BSA)的质量比为1：100)、低的检测下限(10fmol)、大的结合容量(200 mg/g)和高的浓缩回收率(85%以上)等显著优点。新型Fe3O4-PEI-pMaltose纳米粒子的优异性能归功于麦芽糖聚合物刷子的接枝和高效的组装策略。进一步利用Fe3O4-PEI-p麦芽糖纳米粒选择性富集人肾小球系膜细胞糖肽，经200μg胰酶消化，鉴定出449个N-连接的糖肽，分别代表323种不同的糖蛋白和476个糖基化位点。预计合成的Fe3O4-PEI-pMaltose纳米粒子具有良好的性能(高结合容量、良好的选择性、低检测限、高浓缩回收率和易于磁分离)，在复杂生物样品的N-糖基化蛋白质组分析中具有巨大的潜力。
Efficient enrichment glycoproteins/glycopeptides from complex biological solutions are very important in the biomedical sciences, in particular biomarker research. In this work, the high hydrophilic polyethylenimine conjugated polymaltose polymer brushes functionalized magnetic Fe3O4 nanoparticles (NPs) denoted as Fe3O4–PEI–pMaltose were designed and synthesized via a simple two-step modification. The obtained superhydrophilic Fe3O4–PEI–pMaltose NPs displayed outstanding advantages in the enrichment of N-linked glycopeptides, including high selectivity (1:100, mass ratios of HRP and bovine serum albumin (BSA) digest), low detection limit (10 fmol), large binding capacity (200 mg/g), and high enrichment recovery (above 85%). The above-mentioned excellent performance of novel Fe3O4–PEI–pMaltose NPs was attributed to graft of maltose polymer brushes and efficient assembly strategy. Moreover, Fe3O4–PEI–pMaltose NPs were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 449 N-linked glycopeptides, representing 323 different glycoproteins and 476 glycosylation sites, were identified. It was expected that the as-synthesized Fe3O4–PEI–pMaltose NPs, possessing excellent performance (high binding capacity, good selectivity, low detection limit, high enrichment recovery, and easy magnetic separation) coupled to a facile preparation procedure, have a huge potential in N-glycosylation proteome analysis of complex biological samples.
登录
查看更多内容
用于高选择性富集糖肽的介孔聚合物的无模板合成
影响因子：
7.015
作者：
Jin, Tian;Xiong, Zhichao;Dai, Sheng
通讯作者：
Dai, Sheng
轻松合成硼酸功能化磁性碳纳米管，用于高度特异性富集糖肽
影响因子：
6.7
作者：
Ma, Rongna;Hu, Junjie;Ju, Huangxian
通讯作者：
Ju, Huangxian
用于高度特异性富集 N-糖肽的酰肼功能化核壳磁性纳米复合材料
影响因子：
9.5
作者：
Liu, Liting;Yu, Meng;Lu, Haojie
通讯作者：
Lu, Haojie
影响因子：
56.9
作者：
Partridge, EA;Le Roy, C;Dennis, JW
通讯作者：
Dennis, JW
影响因子：
7.4
作者：
Baycin-Hizal, Deniz;Tian, Yuan;Akan, Ilhan;Jacobson, Elena;Clark, Dean;Wu, Alexander;Jampol, Russell;Palter, Karen;Betenbaugh, Michael;Zhang, Hui
通讯作者：
Zhang, Hui