Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells.
Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells.
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DOI：
10.3390/cells10092491
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发表时间：
2021-09-21
期刊：
影响因子：
6
通讯作者：
Maeda H
中科院分区：
文献类型：
--
作者：
Ipposhi K;Tomokiyo A;Ono T;Yamashita K;Alhasan MA;Hasegawa D;Hamano S;Yoshida S;Sugii H;Itoyama T;Ogawa M;Maeda H
直接盖髓是一种有效的治疗方法，通过成牙本质细胞样细胞形成保护性修复性牙本质来保护牙髓免受龋坏或创伤性牙髓暴露。修复性牙本质的形成可以通过几种信号分子刺激，因此，我们研究了分泌型卷曲相关蛋白（SFRP）1的影响，据报道，在成牙本质细胞的新生磨牙牙胚成牙本质细胞分化和修复性牙本质的形成强烈表达。在发育中的大鼠切牙，牙髓细胞，颈环，和内釉上皮细胞，以及成釉细胞和preodontoblasts，弱表达Sfrp 1，然而，Sfrp 1强烈表达在成熟的成牙本质细胞。与牙周膜细胞和牙龈细胞相比，人牙髓细胞（hDPC）中SFRP 1的表达更强。hDPC中SFRP 1的敲低消除了氯化钙诱导的矿化结节形成和成牙本质细胞相关基因表达，并降低了BMP-2基因表达。相反，SFRP 1刺激增强结节形成和BMP-2的表达。直接盖髓治疗与SFRP 1诱导形成了相当数量的修复性牙本质，具有类似于原始牙本质的结构。我们的研究结果表明，SFRP 1是至关重要的牙本质形成，并在促进修复性牙本质的形成，以响应损伤是重要的。
Direct pulp capping is an effective treatment for preserving dental pulp against carious or traumatic pulp exposure via the formation of protective reparative dentin by odontoblast-like cells. Reparative dentin formation can be stimulated by several signaling molecules; therefore, we investigated the effects of secreted frizzled-related protein (SFRP) 1 that was reported to be strongly expressed in odontoblasts of newborn molar tooth germs on odontoblastic differentiation and reparative dentin formation. In developing rat incisors, cells in the dental pulp, cervical loop, and inner enamel epithelium, as well as ameloblasts and preodontoblasts, weakly expressed Sfrp1; however, Sfrp1 was strongly expressed in mature odontoblasts. Human dental pulp cells (hDPCs) showed stronger expression of SFRP1 compared with periodontal ligament cells and gingival cells. SFRP1 knockdown in hDPCs abolished calcium chloride-induced mineralized nodule formation and odontoblast-related gene expression and decreased BMP-2 gene expression. Conversely, SFRP1 stimulation enhanced nodule formation and expression of BMP-2. Direct pulp capping treatment with SFRP1 induced the formation of a considerable amount of reparative dentin that has a structure similar to primary dentin. Our results indicate that SFRP1 is crucial for dentinogenesis and is important in promoting reparative dentin formation in response to injury.
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影响因子：
5.3
作者：
Couve E;Schmachtenberg O
通讯作者：
Schmachtenberg O
DOI：
10.4103/0972-0707.128034
发表时间：
2014-03
期刊：
Journal of conservative dentistry : JCD
影响因子：
--
作者：
Khasnis SA;Kidiyoor KH;Patil AB;Kenganal SB
通讯作者：
Kenganal SB
影响因子：
4.2
作者：
Al-Hezaimi, Khalid;Salameh, Ziad;Tay, Franklin R.
通讯作者：
Tay, Franklin R.
影响因子：
4.6
作者：
Li, Jingyuan;Huang, Xiaofeng;Chai, Yang
通讯作者：
Chai, Yang
影响因子：
--
作者：
Bodine, PVN;Zhao, WG;Komm, BS
通讯作者：
Komm, BS