Osteopontin-induced lncRNA HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.
Osteopontin-induced lncRNA HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.
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骨桥蛋白诱导的 lncRNA HOTAIR 表达通过调节细胞增殖参与骨关节炎
DOI：
10.1186/s12877-020-01993-y
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发表时间：
2021-01-14
期刊：
影响因子：
4.1
通讯作者：
Fang R
中科院分区：
文献类型：
--
作者：
Liang Q;Asila A;Deng Y;Liao J;Liu Z;Fang R
骨桥蛋白通过调节破骨细胞的功能在骨关节炎中发挥重要作用。已知骨桥蛋白可诱导lncRNA HOX转录反义RNA(HOTAIR)的表达，提示HOTAIR参与了骨性关节炎的发病过程。本研究旨在探讨HOTAIR在骨性关节炎中的作用。从骨性关节炎患者(n = 58例)和健康对照(n = 58例)提取滑液。RT-qPCR法检测滑液中骨桥蛋白和HOTAIR的表达。以0、1、5、10µg/ml的骨桥蛋白处理软骨细胞，RT-qPCR检测HOTAIR的表达。CCK-8法分析骨桥蛋白、HOTAIR过表达及HOTAIR基因敲除在调控软骨细胞增殖中的作用。HOTAIR在骨性关节炎中表达上调。HOTAIR与骨桥蛋白呈正相关。在原代软骨细胞中，骨桥蛋白处理增加了HOTAIR的表达，而HOTAIR的过度表达和基因敲除并没有显著影响骨桥蛋白的表达。此外，骨桥蛋白和HOTAIR的过表达促进了软骨细胞的增殖，而HOTAIRE基因敲除则抑制了软骨细胞的增殖。此外，HOTAIR基因敲除降低了骨桥蛋白对细胞增殖的影响。骨桥蛋白诱导的HOTAIR表达通过调节细胞增殖参与骨关节炎的发生。
Osteopontin plays critical roles in osteoarthritis (OA) by regulating the functions of osteoclasts. It is known that osteopontin can induce the expression of lncRNA HOX transcript antisense RNA (HOTAIR), indicating the involvement of HOTAIR in OA. This study was carried out to investigate the role of HOTAIR in OA. Synovial fluid was extracted from both OA patients (n = 58) and healthy controls (n = 58). Expression of osteopontin and HOTAIR in synovial fluid was determined by RT-qPCR. Osteopontin was used to treat chondrocytes at dosages of 0, 1, 5 and 10 µg/ml, followed by measurement of HOTAIR expression by RT-qPCR. The role of osteopontin and HOTAIR overexpression, as well as HOTAIR knockdown in regulating the proliferation of chondrocytes was analyzed by cck-8 assay. HOTAIR was upregulated in OA. A positive correlation between HOTAIR and osteopontin was observed. In the primary chondrocytes, osteopontin treatment increased HOTAIR expression, while HOTAIR overexpression and knockdown failed to significantly affect osteopontin expression. In addition, osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIRE knockdown decreased chondrocyte proliferation. In addition, HOTAIR knockdown reduced the effects of osteopontin treatment on cell proliferation. Osteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.
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影响因子：
64.8
作者：
通讯作者：
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影响因子：
3.7
作者：
Shen, Huijun;Wang, Yue;Zhang, Ying
通讯作者：
Zhang, Ying
影响因子：
168.9
作者：
Glyn-Jones, S.;Palmer, A. J. R.;Carr, A. J.
通讯作者：
Carr, A. J.
DOI：
10.1016/j.bbrc.2018.07.015
发表时间：
2018-09-18
影响因子：
3.1
作者：
Li, Lei;Lv, Guohua;Kuang, Lei
通讯作者：
Kuang, Lei
DOI：
10.1016/j.berh.2009.08.005
发表时间：
2010-02-01
影响因子：
5.2
作者：
Valdes, Ana M.;Spector, Tim D.
通讯作者：
Spector, Tim D.