Effects of aminooxy analogues of biogenic polyamines on aggregation and stability of calf thymus DNA.
Effects of aminooxy analogues of biogenic polyamines on aggregation and stability of calf thymus DNA.
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生物多胺的氨氧基类似物对小牛胸腺 DNA 聚集和稳定性的影响。
DOI：
10.1002/bip.10187
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Thomas,T
中科院分区：
文献类型：
--
作者：
Ramirez,FJ;Thomas,TJ;Antony,T;Ruiz-Chica,J;Thomas,T
研究了生物源性多胺亚精胺和精胺的一系列氨基类似物对小牛胸腺DNA构象的影响。这些新分子是等构和电荷不足的类似物，适合研究生物源多胺分子生理学中电荷分布和结构需求的作用。它们也被证明是抑制多胺生物合成和细胞生长的有用工具。记录了DNA及其氨基类似物溶液在不同浓度（100 ~ 1000 μM）、不同pH值（5 ~ 7.5）下的圆二色性（CD）光谱。我们使用声纳和高度聚合的小牛胸腺DNA。超声DNA的CD光谱显示，在氨基类似物的存在下，形成了类似于液晶的高度有序聚集结构Ψ‐DNA。当多胺浓度增加时，精胺的氨基衍生物引起的聚集随后是DNA崩溃。对于高度聚合的DNA，无法检测到Ψ‐DNA的特征。温度熔化测量支持骨料高度的结构秩序。CD实验表明，指示不能引起DNA大分子结构的重大变化。此外，只有当三亚甲基部分存在于两个相邻的正电荷之间时，才会观察到聚集。不同氨基亚精胺衍生物DNA溶液的CD谱差异表明，亚精胺的不同氨基在与DNA相互作用时所起的作用不同。本研究结果支持了氨基类似物可作为生物源性多胺生理功能研究的良好模型。©2002 Wiley期刊公司生物工程学报（自然科学版），32 (5):559 - 557,2002
The effect of a series of aminooxy analogues of the biogenic polyamines spermidine and spermine on the conformation of calf thymus DNA is studied. These new molecules are isosteric and charge insufficient analogues that are suitable to study the roles of both charge distribution and structural requirements in the molecular physiology of the biogenic polyamines. They are also evidenced as useful tools to inhibit polyamine biosynthesis and cell growth. Circular dichroism (CD) spectra of solutions containing DNA and the aminooxy analogues at different concentrations (100–1000 μM) and different pH values, (5–7.5) are recorded. We use both sonicated and highly polymerized calf thymus DNA. The CD spectra of sonicated DNA showed the formation of Ψ‐DNA, a highly ordered aggregated structure similar to liquid crystals, in the presence of the aminooxy analogues. Aggregation induced by an aminooxy derivative of spermine is followed by DNA collapse when increasing the polyamine concentration. The features of Ψ‐DNA are not detected for highly polymerized DNA. Temperature melting measurements support a high degree of structural order of the aggregates. The CD experiments indicate that dications are unable to induce major changes on the macromolecular structure of DNA. In addition, aggregation is only observed when the trimethylene moiety is present between two adjacent positive charges. The observed differences among the CD spectra of DNA solutions with different aminooxy derivatives of spermidine indicate different roles for different amino groups of this biogenic polyamine when interacting with DNA. Our results support the idea that aminooxy analogues can be used as good models in studying the physiological functions of biogenic polyamines. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 65: 148–157, 2002
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影响因子：
2.9
作者：
Saminathan, M;Antony, T;Thomas, TJ
通讯作者：
Thomas, TJ
水溶液中脱氧核糖核酸的超声断裂 I. 超声处理条件和超声处理样品的分子量
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
K. Fukudome;K. Yamaoka;Kenichiro Nishikori;Takamichi Takahashi;O. Yamamoto
通讯作者：
O. Yamamoto
影响因子：
2.9
作者：
PHILLIPS, CL;MICKOLS, WE;TINOCO, I
通讯作者：
TINOCO, I
影响因子：
11.2
作者：
Anthony E. Pegg
通讯作者：
Anthony E. Pegg
精胺对 DNA 的辐射防护：一种分子建模方法。
影响因子：
2.6
作者：
D. Sy;S. Hugot;C. Savoye;S. Ruiz;M. Charlier;M. Spotheim
通讯作者：
M. Spotheim