Evolutionary origins, molecular cloning and expression of carotenoid hydroxylases in eukaryotic photosynthetic algae.
Evolutionary origins, molecular cloning and expression of carotenoid hydroxylases in eukaryotic photosynthetic algae.
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真核光合藻类类胡萝卜素羟化酶的进化起源、分子克隆和表达
DOI：
10.1186/1471-2164-14-457
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发表时间：
2013-07-08
期刊：
影响因子：
4.4
通讯作者：
Qin S
中科院分区：
文献类型：
--
作者：
Cui H;Yu X;Wang Y;Cui Y;Li X;Liu Z;Qin S
研究背景叶黄素是胡萝卜素的含氧衍生物，在蓝藻、藻类和高等植物的光合机构中起着重要作用。虽然藻类的叶黄素生物合成途径在很大程度上是未知的，但它特别令人感兴趣，因为它们具有非常复杂的进化历史。类胡萝卜素羟化酶（Carotenoid hydroxylase，CHY）是叶黄素生物合成过程中的重要蛋白质。与可用的18个已测序的藻类基因组，我们进行了全面的比较分析ofchygenes，并探讨其分布，结构，进化，起源，和expression.ResultsOverall 60 putativechygenes被确定为两个主要的亚科（bchandcyp 97）根据其结构域的结构。在10种绿色藻类和1种红藻中发现了bch亚家族的基因，而在其它藻类中没有发现。在系统发育树中，绿色藻类和高等植物的bchgenes有共同的祖先，且起源于非蓝藻，而红藻的bchgenes则起源于蓝藻。cyp 97 a/c基因的同源物仅在绿色藻类中广泛存在，而cyp 97 b的旁系同源物在大多数藻类中都存在。对cyp 97基因的系统发育分析支持cyp 97是一个起源于现存藻类类群形成之前的古老基因的假设。cyp 97 agene与cyp 97 cin的进化关系比cyp 97 b更密切。从雨生红球藻（Haematococcus pluvialis）中分离得到两个cyp 97基因，并在不同波长的强光胁迫下观察其转录表达谱。虽然红藻在初级内共生过程中继承了蓝细菌的途径，但在Chromalveolates中仍然不清楚。α-叶黄素生物合成途径是绿色藻类和高等植物的共同特征。cyp 97 a/cis的起源很可能是绿色藻类与高等植物分化前的基因复制所致。绿色藻H蛋白质结构域及其表达分析。结果表明，不同的植物基因对光照的反应方式不同。这些研究为进一步克隆藻类中的凝乳酶基因及其功能研究提供了基础。
BackgroundXanthophylls, oxygenated derivatives of carotenes, play critical roles in photosynthetic apparatus of cyanobacteria, algae, and higher plants. Although the xanthophylls biosynthetic pathway of algae is largely unknown, it is of particular interest because they have a very complicated evolutionary history. Carotenoid hydroxylase (CHY) is an important protein that plays essential roles in xanthophylls biosynthesis. With the availability of 18 sequenced algal genomes, we performed a comprehensive comparative analysis ofchygenes and explored their distribution, structure, evolution, origins, and expression.ResultsOverall 60 putativechygenes were identified and classified into two major subfamilies (bchandcyp97) according to their domain structures. Genes in thebchsubfamily were found in 10 green algae and 1 red alga, but absent in other algae. In the phylogenetic tree,bchgenes of green algae and higher plants share a common ancestor and are of non-cyanobacterial origin, whereas that of red algae is of cyanobacteria. The homologs ofcyp97a/cgenes were widespread only in green algae, whilecyp97bparalogs were seen in most of algae. Phylogenetic analysis oncyp97genes supported the hypothesis thatcyp97bis an ancient gene originated before the formation of extant algal groups. Thecyp97agene is more closely related tocyp97cin evolution than tocyp97b. The twocyp97genes were isolated from the green algaHaematococcus pluvialis, and transcriptional expression profiles ofchygenes were observed under high light stress of different wavelength.ConclusionsGreen algae received aβ-xanthophylls biosynthetic pathway from host organisms. Although red algae inherited the pathway from cyanobacteria during primary endosymbiosis, it remains unclear in Chromalveolates. Theα-xanthophylls biosynthetic pathway is a common feature in green algae and higher plants. The origination ofcyp97a/cis most likely due to gene duplication before divergence of green algae and higher plants. Protein domain structures and expression analyses in green algaH. pluvialisindicate that variouschygenes are in different manners response to light. The knowledge of evolution ofchygenes in photosynthetic eukaryotes provided information of gene cloning and functional investigation ofchygenes in algae in the future.
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影响因子：
2.1
作者：
Cui, Hongli;Wang, Yinchu;Qin, Song
通讯作者：
Qin, Song
影响因子：
2.9
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
通讯作者：
HOCHSTRASSER, D
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
--
作者：
Cunningham, Francis X., Jr.;Lee, Hansel;Gantt, Elisabeth
通讯作者：
Gantt, Elisabeth
影响因子：
4.2
作者：
Becker, Burkhard;Marin, Birger
通讯作者：
Marin, Birger