MicroRNA-9 functions as a tumor suppressor and enhances radio-sensitivity in radio-resistant A549 cells by targeting neuropilin 1.
MicroRNA-9 functions as a tumor suppressor and enhances radio-sensitivity in radio-resistant A549 cells by targeting neuropilin 1.
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MicroRNA-9 作为肿瘤抑制剂发挥作用，并通过靶向神经毡蛋白 1 增强抗辐射 A549 细胞的放射敏感性
DOI：
10.3892/ol.2017.7705
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发表时间：
2018-03
期刊：
影响因子：
2.9
通讯作者：
Xue LX
中科院分区：
文献类型：
--
作者：
Xiong K;Shao LH;Zhang HQ;Jin L;Wei W;Dong Z;Zhu YQ;Wu N;Jin SZ;Xue LX
放射治疗通常用于治疗肺癌，但并不能杀死所有的癌细胞，这可能归因于非小细胞肺癌(NSCLC)经常出现的放射抵抗。目前，辐射抗性的分子机制尚不清楚。NRP1是血管内皮细胞生长因子的辅助受体，在我们先前的研究中，它通过血管内皮生长因子-磷脂酰肌醇-3-激酶-核因子-κB途径与非小细胞肺癌细胞的放射抵抗有关。有人推测，某些microRNAs(MiRs)可能通过调节Nrp1而在辐射敏感性中发挥关键作用。生物信息学预测Nrp1是miR-9的潜在靶点，并通过荧光素酶报告基因分析验证了这一点。在功能上，转miR-9基因的A549细胞的增殖率降低，凋亡率增加，迁移和侵袭能力减弱。此外，miR-9的高表达也显著增强了A549细胞在体外和体内的放射敏感性。这些数据加深了对细胞放射抗性机制的理解，并提示miR-9可能是预测非小细胞肺癌放射敏感性的分子靶点。
Radiotherapy is commonly used to treat lung cancer but may not kill all cancer cells, which may be attributed to the radiotherapy resistance that often occurs in non-small cell lung cancer (NSCLC). At present, the molecular mechanism of radio-resistance remains unclear. Neuropilin 1 (NRP1), a co-receptor for vascular endothelial growth factor (VEGF), was demonstrated to be associated with radio-resistance of NSCLC cells via the VEGF-phosphoinositide 3-kinase-nuclear factor-κB pathway in our previous study. It was hypothesized that certain microRNAs (miRs) may serve crucial functions in radio-sensitivity by regulating NRP1. Bioinformatics predicted that NRP1 was a potential target of miR-9, and this was validated by luciferase reporter assays. Functionally, miR-9-transfected A549 cells exhibited a decreased proliferation rate, increased apoptosis rate and attenuated migratory and invasive abilities. Additionally, a high expression of miR-9 also significantly enhanced the radio-sensitivity of A549 cells in vitro and in vivo. These data improve understanding of the mechanisms of cell radio-resistance, and suggest that miR-9 may be a molecular target for the prediction of radio-sensitivity in NSCLC.
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DOI：
10.1073/pnas.1310144110
发表时间：
2013-07-09
影响因子：
11.1
作者：
Chen, Ping;Price, Colles;Chen, Jianjun
通讯作者：
Chen, Jianjun
DOI：
10.1016/j.bbrc.2012.07.151
发表时间：
2012-09-07
影响因子：
3.1
作者：
Glinka, Yelena;Mohammed, Nada;Prud'homme, Gerald J.
通讯作者：
Prud'homme, Gerald J.
影响因子：
7.3
作者：
Liu, Shujing;Kumar, Suresh M.;Lu, Hezhe;Liu, Aihua;Yang, Ruifeng;Pushparajan, Anitha;Guo, Wei;Xu, Xiaowei
通讯作者：
Xu, Xiaowei
影响因子：
21.3
作者：
Ma, Li;Young, Jennifer;Prabhala, Harsha;Pan, Elizabeth;Mestdagh, Pieter;Muth, Daniel;Teruya-Feldstein, Julie;Reinhardt, Ferenc;Onder, Tamer T.;Valastyan, Scott;Westermann, Frank;Speleman, Frank;Vandesompele, Jo;Weinberg, Robert A.
通讯作者：
Weinberg, Robert A.
影响因子：
254.7
作者：
Parkin, DM;Bray, F;Pisani, P
通讯作者：
Pisani, P