Bioinformatic characterisation of the effector repertoire of the strawberry pathogen Phytophthora cactorum.

Bioinformatic characterisation of the effector repertoire of the strawberry pathogen Phytophthora cactorum.
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DOI:
10.1371/journal.pone.0202305
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发表时间:
2018
期刊:
影响因子:
3.7
通讯作者:
Brurberg MB
Brurberg MB
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Armitage AD;Lysøe E;Nellist CF;Lewis LA;Cano LM;Harrison RJ;Brurberg MB

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The oomycete pathogen Phytophthora cactorum causes crown rot, a major disease of cultivated strawberry. We report the draft genome of P. cactorum isolate 10300, isolated from symptomatic Fragaria x ananassa tissue. Our analysis revealed that there are a large number of genes encoding putative secreted effectors in the genome, including nearly 200 RxLR domain containing effectors, 77 Crinklers (CRN) grouped into 38 families, and numerous apoplastic effectors, such as phytotoxins (PcF proteins) and necrosis inducing proteins. As in other Phytophthora species, the genomic environment of many RxLR and CRN genes differed from core eukaryotic genes, a hallmark of the two-speed genome. We found genes homologous to known Phytophthora infestans avirulence genes including Avr1, Avr3b, Avr4, Avrblb1 and AvrSmira2 indicating effector sequence conservation between Phytophthora species of clade 1a and clade 1c. The reported P. cactorum genome sequence and associated annotations represent a comprehensive resource for avirulence gene discovery in other Phytophthora species from clade 1 and, will facilitate effector informed breeding strategies in other crops.
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