Effects of Fatty-Type and Lean-Type on Growth Performance and Lipid Droplet Metabolism in Pekin Ducks.
Effects of Fatty-Type and Lean-Type on Growth Performance and Lipid Droplet Metabolism in Pekin Ducks.
复制标题
肥型和瘦型对北京鸭生长性能和脂滴代谢的影响
DOI：
10.3390/ani12172268
复制
发表时间：
2022-09-01
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
动物体内的脂肪沉积与脂肪合成代谢密切相关。为了进一步探讨不同代谢类型的差异是如何调节脂肪沉积的，我们比较了脂肪型和瘦肉型鸭的生长性能和脂滴代谢。结果表明，饲喂相同日粮后，脂肪型鸭生长速度较快，早期脂肪沉积较多，在血清和脂肪组织中产生较多的脂蛋白。然而，肝脏中积累的甘油三酯较少。我们认为，脂肪型鸭的这种表现是由于脂滴相关基因表达水平的提高所致。脂肪型(F-T)鸭和瘦肉型(L-T)鸭脂肪沉积差异的原因尚不清楚。本研究旨在比较F-T和L-T北京鸭的生长性能、脂肪沉积和与脂滴形成相关的基因表达。选用1日龄L-T公鸭和F-T公鸭各140只，分成20个重复。所有鸭均饲喂商品饲料至35日龄。F-T鸭在21~28日龄有较高的日增重。35日龄时，F-T鸭血清高密度脂蛋白、低密度脂蛋白、胆固醇、白蛋白和羟丁酸脱氢酶活性均高于L-T鸭。F-T鸭的腹脂率和皮下脂肪率均高于L-T鸭。肝脏组织学检查显示，L-T鸭肝脏中含有较多的脂滴，随着日龄的增加，脂滴含量逐渐减少。F-T鸭和L-T鸭腹脂和皮下脂肪的平均脂肪细胞面积和直径随年龄增长而增大，且F-T鸭高于L-T鸭。此外，F-T鸭肝脏、腹脂和皮下脂肪中Perilipin 1、Perilipin 2、血管生成素样蛋白4、脂肪甘油三酯脂肪酶、α/β-水解酶结构域蛋白5和丝氨酸/苏氨酸激酶17a的基因表达水平均高于L-T鸭，且随日龄增加而增加。与L-T鸭相比，F-T鸭腹脂和皮下脂肪中ABHD5的表达较高，肝脏中的表达较低。因此，F-T鸭表现出较低的肝脏脂肪沉积和较高的腹脂和皮下脂肪百分比，这表明F-T鸭具有较高的脂肪储存能力，这是由于与脂滴相关的基因表达增加所致。
Lipid deposition in animals is closely related to lipid anabolism. In order to further explore how differences in different metabolic types regulate lipid deposition, we compared the growth performance and lipid droplet metabolism of fatty-type ducks and lean-type ducks. The results showed that fatty-type ducks showed a faster growth rate and more fat deposition in the early growth stage after feeding the same diet, and produced more lipoproteins in serum and deposited in adipose tissue. However, fewer triglycerides accumulated in the liver. We believe that this performance of fatty-type ducks is caused by the increased expression level of lipid droplet-related genes. The reasons for differences in lipid depositions between fatty-type (F-T) and lean-type (L-T) ducks remain unknown. The present study aimed to compare the growth performance, lipid deposition, and gene expression related to lipid droplet formation in F-T and L-T Pekin ducks. One-day-old, 140 each L-T and F-T male ducks were selected and distributed separately into 20 replicate cages. All ducks were fed commercial diets up to 35 d of age. F-T ducks had a higher average daily gain from 21 to 28 d of age. On 35-day-old, F-T ducks had higher serum levels of high- and low-density lipoprotein cholesterol, cholesterol, albumin, and hydroxybutyrate dehydrogenase activity than L-T ducks. F-T ducks had higher abdominal fat and subcutaneous fat percentages than those in L-T ducks. Liver histological examination showed that L-T ducks contained more lipid droplets in the liver, which gradually decreased with increasing age. The average adipocyte area and diameter of abdominal fat and subcutaneous fat in the F-T and L-T ducks increased with age and were higher in F-T ducks than those in L-T ducks. Furthermore, the gene expression of perilipin 1, perilipin 2, angiopoietin-like protein 4, adipose triglyceride lipase, alpha/beta-hydrolase domain-containing protein 5 (ABHD5), and serine/threonine kinase 17a in the liver, abdominal fat, and subcutaneous fat of F-T ducks was higher than that in L-T ducks, and it increased with age. Compared to L-T ducks, F-T ducks had higher expression of ABHD5 in the abdominal fat and subcutaneous fat and lower expression in the liver. Thus, F-T ducks displayed lower hepatic lipid deposition and a higher percentage of abdominal fat and subcutaneous fat, suggesting that F-T ducks had higher lipid storage capacity due to increased gene expression related to lipid droplets.
登录
查看更多内容
影响因子：
3.3
作者：
HAUSMAN, GJ;MARTIN, RJ
通讯作者：
MARTIN, RJ
影响因子：
4.2
作者：
HERMIER, D;QUIGNARDBOULANGE, A;LECLERCQ, B
通讯作者：
LECLERCQ, B
影响因子：
11.1
作者：
Krahmer, Natalie;Farese, Robert V., Jr.;Walther, Tobias C.
通讯作者：
Walther, Tobias C.
遗传选择和苏氨酸对北京鸭肉品质的影响
影响因子：
4.4
作者：
Jiang Y;Xie M;Tang J;Zhou Z;Zhang Y;Chen G;Hou S
通讯作者：
Hou S
影响因子：
7.7
作者：
Bell, Ming;Wang, Hong;Chen, Hui;McLenithan, John C.;Gong, Da-Wei;Yang, Rong-Zee;Yu, Daozhan;Fried, Susan K.;Quon, Michael J.;Londos, Constantine;Sztalryd, Carole
通讯作者：
Sztalryd, Carole