Photonic crystal protein hydrogel sensor materials enabled by conformationally induced volume phase transition.
Photonic crystal protein hydrogel sensor materials enabled by conformationally induced volume phase transition.
复制标题
DOI：
10.1039/c6sc00682e
复制
发表时间：
2016-07-01
期刊：
影响因子：
8.4
通讯作者：
Asher SA
中科院分区：
文献类型：
--
作者：
Cai Z;Luck LA;Punihaole D;Madura JD;Asher SA
我们利用蛋白质构象变化诱导的体积相变，制备了葡萄糖/半乳糖结合蛋白传感水凝胶用于葡萄糖检测。根据特定分子刺激而改变体积的水凝胶可以作为传感器、执行器和药物输送装置的平台。人们对利用蛋白质结合特性和构象变化设计用于组织工程、药物输送和微流体的智能水凝胶非常感兴趣。配基结合引起的蛋白质构象变化会引起体积相变(Vpt)。在这里，我们开发了一种高选择性的葡萄糖传感蛋白光子晶体(PC)水凝胶，它是由基因工程的大肠杆菌葡萄糖/半乳糖结合蛋白(GGBP)制成的。由此得到的2-D PC-GGBP水凝胶在葡萄糖的响应下经历VPT。体积变化导致2-D PC阵列颗粒间距减小，导致蓝移衍射，使我们的传感器能够报告葡萄糖浓度。这种2-D PC-GGBP响应型水凝胶是一种选择性和灵敏的传感器，可以轻松监测从∼0.2μM到∼10 mM的血糖浓度。这项工作展示了一种利用配体结合诱导蛋白质构象变化的VPTS开发响应性的、“智能的”蛋白质水凝胶材料的概念验证。这一创新可能会使其他新型化学传感器和高通量筛选设备的开发成为可能，这些设备可以监测蛋白质与药物的结合作用。
We fabricated photonic crystal glucose/galactose binding protein sensing hydrogels for glucose detection using protein conformational change induced volume phase transition. Hydrogels that change volume in response to specific molecular stimuli can serve as platforms for sensors, actuators and drug delivery devices. There is great interest in designing intelligent hydrogels for tissue engineering, drug delivery, and microfluidics that utilize protein binding specificities and conformational changes. Protein conformational change induced by ligand binding can cause volume phase transitions (VPTs). Here, we develop a highly selective glucose sensing protein photonic crystal (PC) hydrogel that is fabricated from genetically engineered E. coli glucose/galactose binding protein (GGBP). The resulting 2-D PC-GGBP hydrogel undergoes a VPT in response to glucose. The volume change causes the 2-D PC array particle spacing to decrease, leading to a blue-shifted diffraction which enables our sensors to report on glucose concentrations. This 2-D PC-GGBP responsive hydrogel functions as a selective and sensitive sensor that easily monitors glucose concentrations from ∼0.2 μM to ∼10 mM. This work demonstrates a proof-of-concept for developing responsive, “smart” protein hydrogel materials with VPTs that utilize ligand binding induced protein conformational changes. This innovation may enable the development of other novel chemical sensors and high-throughput screening devices that can monitor protein–drug binding interactions.
登录
查看更多内容
影响因子：
29.4
作者：
Ehrick, Jason D.;Stokes, Sean;Daunert, Sylvia
通讯作者：
Daunert, Sylvia
影响因子：
4.6
作者：
Ehrick, Jason D.;Luckett, Matthew R.;Daunert, Sylvia
通讯作者：
Daunert, Sylvia
影响因子：
24.2
作者：
Ionov, Leonid
通讯作者：
Ionov, Leonid
影响因子：
16.6
作者：
Murphy, William L.;Dillmore, W. Shannon;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
56.9
作者：
VYAS, NK;VYAS, MN;QUIOCHO, FA
通讯作者：
QUIOCHO, FA