Construction of a food-grade cell surface display system for Lactobacillus casei.
Construction of a food-grade cell surface display system for Lactobacillus casei.
复制标题
食品级干酪乳杆菌细胞表面展示系统的构建
DOI：
10.1016/j.micres.2014.02.001
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发表时间：
2014-09
影响因子：
6.7
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Qin J;Wang X;Kong J;Ma C;Xu P
本研究构建了食品级干酪乳杆菌细胞表面展示宿主/载体系统。食品级寄主L. caseiQ-5是L.通过质粒消除获得的干酪素ATCC 334。以乳酸菌的安全DNA元件为基础构建了食品级细胞表面展示载体，该载体含有乳酸乳球菌的pSH 71复制子、乳酸乳球菌的乳糖代谢基因和乳酸乳球菌的乳糖代谢基因。casei ATCC 334作为互补标记，嗜酸乳杆菌ATCC 4356的表层蛋白基因用于细胞表面展示。通过绿色荧光蛋白在L. casei。激光扫描共聚焦显微镜和免疫荧光分析使用抗GFP抗体证实，GFP锚定在重组细胞的表面。重组L.在人工胃肠道条件下，证实了干酪素细胞，这对于口服疫苗接种应用是有益的。这些结果表明，食品级宿主/载体系统可以是一个很好的抗原载体口服疫苗的建设。
In this study, a food-grade cell surface display host/vector system for Lactobacillus casei was constructed. The food-grade host L. casei Q-5 was a lactose-deficient derivative of L. casei ATCC 334 obtained by plasmid elimination. The food-grade cell surface display vector was constructed based on safe DNA elements from lactic acid bacteria containing the following: pSH71 replicon from Lactococcus lactis, lactose metabolism genes from L. casei ATCC 334 as complementation markers, and surface layer protein gene from Lactobacillus acidophilus ATCC 4356 for cell surface display. The feasibility of the new host/vector system was verified by the expression of green fluorescent protein (GFP) on L. casei. Laser scanning confocal microscopy and immunofluorescence analysis using anti-GFP antibody confirmed that GFP was anchored on the surface of the recombinant cells. The stability of recombinant L. casei cells in artificial gastrointestinal conditions was verified, which is beneficial for oral vaccination applications. These results indicate that the food-grade host/vector system can be an excellent antigen delivery vehicle in oral vaccine construction.
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影响因子：
5.5
作者：
Kajikawa A;Satoh E;Leer RJ;Yamamoto S;Igimi S
通讯作者：
Igimi S
影响因子：
5.5
作者：
Reveneau, N;Geoffroy, MC;Mercenier, A
通讯作者：
Mercenier, A
影响因子：
3.2
作者：
Smit, E;Pouwels, PH
通讯作者：
Pouwels, PH
影响因子：
5.6
作者：
Smit, E;Oling, F;Pouwels, PH
通讯作者：
Pouwels, PH
DOI：
10.1111/j.1365-2672.1960.tb00188.x
发表时间：
1960-01-01
期刊：
JOUR APPL BACT
影响因子：
--
作者：
DE MAN, J. C.;ROGOSA, M.;SHARPE, M. ELIZABETH
通讯作者：
SHARPE, M. ELIZABETH