No monkey business: why studying NK cells in non-human primates pays off.
No monkey business: why studying NK cells in non-human primates pays off.
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DOI：
10.3389/fimmu.2013.00032
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发表时间：
2013
影响因子：
7.3
通讯作者：
Johnson RP
中科院分区：
文献类型：
--
作者：
Hong HS;Rajakumar PA;Billingsley JM;Reeves RK;Johnson RP
人NK细胞在介导宿主免疫应答中起着重要作用。出于实际原因，大多数关于hNK细胞的数据是使用外周血淋巴细胞生成的。相反，我们对人体组织中NK细胞的了解是有限的，并且对hNK细胞亚群在体内的发育途径知之甚少。尽管小鼠研究已经阐明了NK细胞生物学的许多基本特征，但小鼠和hNK细胞在亚群、功能和受体库方面存在显著差异。因此，需要一种与人类更密切相关但又允许实验操作的模型。非人灵长类动物模型为NK细胞的研究提供了许多机会，包括研究实体器官和干细胞移植后NK细胞的作用，以及在急性病毒感染中的作用。猕猴NK细胞可以在体内耗尽或在自体系统中过继转移。所有这些研究在人类身上进行要么是困难的，要么是不道德的。在这里，我们强调了恒河猴NK细胞研究的最新进展及其在人类中的相似之处。使用高通量的转录谱，我们证明了人类CD56bright和CD56dim NK细胞亚群在恒河猴中具有表型和功能相似的对应物。因此，使用非人灵长类动物模型提供了实质性推进hNK细胞研究的潜力。
Human NK (hNK) cells play a key role in mediating host immune responses against various infectious diseases. For practical reasons, the majority of the data on hNK cells has been generated using peripheral blood lymphocytes. In contrast, our knowledge of NK cells in human tissues is limited, and not much is known about developmental pathways of hNK cell subpopulations in vivo. Although research in mice has elucidated a number of fundamental features of NK cell biology, mouse, and hNK cells significantly differ in their subpopulations, functions, and receptor repertoires. Thus, there is a need for a model that is more closely related to humans and yet allows experimental manipulations. Non-human primate models offer numerous opportunities for the study of NK cells, including the study of the role of NK cells after solid organ and stem cell transplantation, as well as in acute viral infection. Macaque NK cells can be depleted in vivo or adoptively transferred in an autologous system. All of these studies are either difficult or unethical to carry out in humans. Here we highlight recent advances in rhesus NK cell research and their parallels in humans. Using high-throughput transcriptional profiling, we demonstrate that the human CD56bright and CD56dim NK cell subsets have phenotypically and functionally analogous counterparts in rhesus macaques. Thus, the use of non-human primate models offers the potential to substantially advance hNK cell research.
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影响因子：
64.8
作者：
Cella, Marina;Fuchs, Anja;Vermi, William;Facchetti, Fabio;Otero, Karel;Lennerz, Jochen K. M.;Doherty, Jason M.;Mills, Jason C.;Colonna, Marco
通讯作者：
Colonna, Marco
影响因子：
5.4
作者：
Hong, Henoch S.;Eberhard, Johanna M.;Meyer-Olson, Dirk
通讯作者：
Meyer-Olson, Dirk
影响因子：
5.4
作者：
Fogli, M;Costa, P;De Maria, A
通讯作者：
De Maria, A
影响因子：
100.3
作者：
Eagle, Robert A.;Trowsdale, John
通讯作者：
Trowsdale, John
影响因子：
3.8
作者：
Hong, Henoch S.;Eberhard, Johanna M.;Meyer-Olson, Dirk
通讯作者：
Meyer-Olson, Dirk