Pancreatic PYY but not PPY expression is responsive to short-term nutritional state and the pancreas constitutes the major site of PYY mRNA expression in chickens.
Pancreatic PYY but not PPY expression is responsive to short-term nutritional state and the pancreas constitutes the major site of PYY mRNA expression in chickens.
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胰腺PYY而非PPY表达对短期营养状态有反应，胰腺构成了PYY mRNA表达的主要部位。
DOI：
10.1016/j.ygcen.2017.07.002
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发表时间：
2017-10-01
影响因子：
2.7
通讯作者：
Dunn IC
中科院分区：
文献类型：
--
作者：
Reid AMA;Wilson PW;Caughey SD;Dixon LM;D'Eath RB;Sandilands V;Boswell T;Dunn IC
鸡和鹌鹑PYY mRNA序列证明。胰腺是鸡PYY mRNA表达的主要部位。肠表达的峰值在远端空肠周围证实。报道了鸡PYY和PPY胰腺表达梯度的个体发育。鸡胰腺PYY显示出响应于短期营养状态而增加。已知PP折叠肽如肽YY（PYY）和胰多肽（PPY）在脊椎动物能量稳态中起关键作用。直到最近，还没有禽类PYY的基因序列，因此在禽类物种中存在对其表达调控的知识空白。在这里，我们进一步证明了鸡PYY的mRNA序列，并表明胰腺是其mRNA表达的主要位点，在远端空肠周围有第二个表达峰，与大肠是PYY表达的主要位点的哺乳动物相反。我们还表明，胰腺PYY的表达是响应于短期和长期的营养状态，增加数小时内喂养，在肠道PYY不波动到相同的程度，和胰腺PPY，这似乎主要是由长期的能量状态。胰腺PYY和PPY的表达被发现表现出个体发育，在年轻（2周）的小鸡中均匀分布在整个胰腺中，但在青春期（12周）时脾脏到十二指肠的梯度降低。
Chicken and quail PYY mRNA sequences evidenced. Pancreas identified as the major site of PYY mRNA expression in chickens. Peak of intestinal expression evidenced around the distal jejunum. Ontogenic development of gradient pancreatic expression of chicken PYY and PPY reported. Chicken pancreatic PYY shown to increase in response to short-term nutritional state. PP-fold peptides such as peptide YY (PYY) and pancreatic polypeptide (PPY) are known to play key roles in vertebrate energy homeostasis. Until recently, no gene sequence was available for avian PYY and therefore a gap in knowledge of regulation of its expression exists in avian species. Here we further evidence the mRNA sequence for chicken PYY and show that the pancreas is the major site of its mRNA expression, with a secondary peak of expression around the distal jejunum, in contrast to mammals where the large intestine is the major site of PYY expression. We also demonstrate that pancreatic PYY expression is responsive to short-term and long-term nutritional state, increasing within hours of feeding, in contrast to intestinal PYY which does not fluctuate to the same extent, and pancreatic PPY which appears to be primarily determined by long-term energy state. Both pancreatic PYY and PPY expression were found to exhibit ontogeny, being evenly distributed throughout the pancreas in young (2wk) chicks but having a decreasing splenic to duodenal gradient by adolescence (12wk).
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DOI：
10.1139/bcb-78-3-371
发表时间：
2000-01-01
期刊：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
影响因子：
--
作者：
Cerdá-Reverter, JM;Larhammar, D
通讯作者：
Larhammar, D
影响因子：
3
作者：
Boey, Dana;Sainsbury, Amanda;Herzog, Herbert
通讯作者：
Herzog, Herbert
影响因子：
1.9
作者：
Coles, BA;Croom, J;Eisen, EJ
通讯作者：
Eisen, EJ
影响因子：
3.5
作者：
CONLON, JM;OHARTE, F
通讯作者：
OHARTE, F
影响因子：
4.7
作者：
Holmberg, SKS;Mikko, S;Larhammar, D
通讯作者：
Larhammar, D