Plasma-derived MHC class II+ exosomes from tumor-bearing mice suppress tumor antigen-specific immune responses.
Plasma-derived MHC class II+ exosomes from tumor-bearing mice suppress tumor antigen-specific immune responses.
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DOI：
10.1002/eji.201141978
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发表时间：
2012-07
影响因子：
5.4
通讯作者：
Robbins, Paul D.
中科院分区：
文献类型：
--
作者：
Yang, Chenjie;Ruffner, Melanie A.;Kim, Seon-Hee;Robbins, Paul D.
肿瘤特异性免疫抑制经常在肿瘤宿主中观察到。外泌体是由大多数肿瘤和造血细胞分泌的纳米尺寸的、内体衍生的膜囊泡，并且已被证明积极参与免疫调节。我们先前证明，抗原特异性免疫抑制外泌体可以从抗原免疫小鼠的血浆中分离。在这里，我们证明了从携带OVA表达肿瘤的小鼠中分离的血浆来源的外泌体能够在小鼠迟发型超敏反应模型中抑制OVA特异性免疫应答。使用外泌体标记方法未检测到携带皮下黑素瘤的小鼠的血浆中肿瘤来源的外泌体的富集。相反，从血浆来源的外泌体中耗尽MHC II类+囊泡或使用从MHC II类缺陷小鼠分离的血浆来源的外泌体导致抑制作用的显著消除。这些结果表明，荷瘤宿主中循环宿主来源的MHC II类+外泌体能够抑制对肿瘤抗原特异性的免疫应答。
Tumor-specific immunosuppression is frequently observed in tumor-bearing hosts. Exosomes are nano-sized, endosomal-derived membrane vesicles secreted by most tumor and hematopoietic cells and have been shown to actively participate in immune regulation. We previously demonstrated that antigen-specific immunosuppressive exosomes could be isolated from the blood plasma of antigen-immunized mice. Here we demonstrate that plasma-derived exosomes isolated from mice bearing OVA-expressing tumors were able to suppress OVA-specific immune response in a mouse delayed-type hypersensitivity model. Enrichment of tumor-derived exosomes in the plasma of mice bearing subcutaneous melanoma was not detected using an exosome-tagging approach. Instead, depletion of MHC Class II+ vesicles from plasma-derived exosomes or using plasma-derived exosomes isolated from MHC Class II deficient mice resulted in significant abrogation of the suppressive effect. These results demonstrate that circulating host-derived, MHC Class II+ exosomes in tumor-bearing hosts are able to suppress the immune response specific to tumor antigens.
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影响因子：
3.8
作者：
Li J;Sherman-Baust CA;Tsai-Turton M;Bristow RE;Roden RB;Morin PJ
通讯作者：
Morin PJ
影响因子：
4.4
作者：
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Batanero, Eva
影响因子：
3.7
作者：
Karlsson, MR;Kahu, H;Dahlgren, UI
通讯作者：
Dahlgren, UI
影响因子：
3.7
作者：
Yang C;Kim SH;Bianco NR;Robbins PD
通讯作者：
Robbins PD
影响因子：
21.3
作者：
Skog, Johan;Wuerdinger, Tom;van Rijn, Sjoerd;Meijer, Dimphna H.;Gainche, Laura;Sena-Esteves, Miguel;Curry, William T., Jr.;Carter, Bob S.;Krichevsky, Anna M.;Breakefield, Xandra O.
通讯作者：
Breakefield, Xandra O.