Investigating the Responses of Human Epithelial Cells to Predatory Bacteria.
Investigating the Responses of Human Epithelial Cells to Predatory Bacteria.
复制标题
研究人上皮细胞对掠食性细菌的反应。
DOI：
10.1038/srep33485
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发表时间：
2016-09-15
影响因子：
4.6
通讯作者：
Mitchell RJ
中科院分区：
文献类型：
--
作者：
Monnappa AK;Bari W;Choi SY;Mitchell RJ
用于治疗多重耐药感染的抗生素的一种诱人的替代品是蛭弧菌和类似生物体（BALO），这是一种已知攻击人类病原体的捕食性细菌。因此，在这项研究中，从四个细胞系（三个人和一个小鼠）的反应，其特征在于在暴露于不同的捕食性细菌，蛭弧菌噬菌HD 100，噬菌体BY 1和Bacteriovorax stolpii EB 1。在Raw 264.7小鼠巨噬细胞培养物中，每种捕食者都诱导了TNF-α水平，但与E.杆菌即使后一种菌株以低11.1倍的浓度加入，这也是正确的（p < 0.01）。同样，E.大肠杆菌导致Raw 264.7鼠巨噬细胞活力的显著（54%）损失，而捕食性菌株没有影响。用各种上皮细胞，包括NuLi-1气道，Caco 2，HT 29和T84结肠直肠细胞进行的测试，得到了类似的结果，E. coli诱导产生IL-8。当暴露于捕食者时，NuLi-1和Caco-2细胞的活力略有降低（8%），而T84细胞的活力保持稳定。在任何情况下，捕食性细菌诱导肌动蛋白重排。这些结果清楚地表明了捕食性细菌的温和性质及其对人类细胞的影响。
One beguiling alternative to antibiotics for treating multi-drug resistant infections are Bdellovibrio-and-like-organisms (BALOs), predatory bacteria known to attack human pathogens. Consequently, in this study, the responses from four cell lines (three human and one mouse) were characterized during an exposure to different predatory bacteria, Bdellovibrio bacteriovorus HD100, Bacteriovorus BY1 and Bacteriovorax stolpii EB1. TNF-α levels were induced in Raw 264.7 mouse macrophage cultures with each predator, but paled in comparison to those obtained with E. coli. This was true even though the latter strain was added at an 11.1-fold lower concentration (p < 0.01). Likewise, E. coli led to a significant (54%) loss in the Raw 264.7 murine macrophage viability while the predatory strains had no impact. Tests with various epithelial cells, including NuLi-1 airway, Caco2, HT29 and T84 colorectal cells, gave similar results, with E. coli inducing IL-8 production. The viabilities of the NuLi-1 and Caco-2 cells were slightly reduced (8%) when exposed to the predators, while T84 viability remained steady. In no cases did the predatory bacteria induce actin rearrangement. These results clearly demonstrate the gentle natures of predatory bacteria and their impacts on human cells.
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小鼠铜绿假单胞菌角膜炎：局部噬菌体KPP12给药的影响。
影响因子：
3.7
作者：
Fukuda K;Ishida W;Uchiyama J;Rashel M;Kato S;Morita T;Muraoka A;Sumi T;Matsuzaki S;Daibata M;Fukushima A
通讯作者：
Fukushima A
影响因子：
4.6
作者：
Monnappa AK;Dwidar M;Seo JK;Hur JH;Mitchell RJ
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Mitchell RJ
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4.4
作者：
Kadouri, Daniel E.;Tran, Aimy
通讯作者：
Tran, Aimy
影响因子：
3.1
作者：
ECKMANN, L;KAGNOFF, MF;FIERER, J
通讯作者：
FIERER, J
影响因子：
4.4
作者：
Kadouri, D;O'Toole, GA
通讯作者：
O'Toole, GA