Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
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DOI：
10.1371/journal.pone.0044289
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gilmore BF
中科院分区：
文献类型：
--
作者：
Alkawareek MY;Algwari QT;Laverty G;Gorman SP;Graham WG;O'Connell D;Gilmore BF
在大多数环境中，细菌作为复杂的、有组织的固着细胞群落存在，固着细胞嵌入在被称为生物膜的自产的、水合的细胞外聚合物质的基质中。细菌生物膜代表慢性感染和与使用留置医疗器械（如导管和假体）相关的感染的普遍存在和主要原因。此类感染通常表现出对抗微生物剂、杀生物剂和免疫学攻击的显著增强的耐受性。这使得它们难以，有时不可能，使用常规化疗剂治疗。因此，迫切需要用于预防和根除生物膜相关的慢性和装置相关感染的有效替代方法。大气压非热等离子体作为根除和控制细菌感染和污染的潜在方法正获得越来越多的关注。然而，到目前为止，大多数研究都是针对浮游细菌进行的，而很少关注生物膜生长模式中的细菌。在这项研究中，千赫兹驱动的大气压非热等离子体射流，在氦氧混合物中操作，对铜绿假单胞菌在体外生物膜的活动进行了评估。铜绿假单胞菌生物膜表现出显着的敏感性暴露的等离子体射流流出物，即使是相对较短的曝光时间（10s）。等离子体操作条件的操作，例如，等离子体操作频率，对细菌灭活率有显著影响。存活曲线显示在前60秒内存活细胞的数量快速下降，随后细胞数量减少的速率较慢。通过共聚焦扫描激光显微镜和四唑盐的代谢，XTT，杀菌活性的测量，也证明了等离子体射流的优异的抗生物膜活性。
Bacteria exist, in most environments, as complex, organised communities of sessile cells embedded within a matrix of self-produced, hydrated extracellular polymeric substances known as biofilms. Bacterial biofilms represent a ubiquitous and predominant cause of both chronic infections and infections associated with the use of indwelling medical devices such as catheters and prostheses. Such infections typically exhibit significantly enhanced tolerance to antimicrobial, biocidal and immunological challenge. This renders them difficult, sometimes impossible, to treat using conventional chemotherapeutic agents. Effective alternative approaches for prevention and eradication of biofilm associated chronic and device-associated infections are therefore urgently required. Atmospheric pressure non-thermal plasmas are gaining increasing attention as a potential approach for the eradication and control of bacterial infection and contamination. To date, however, the majority of studies have been conducted with reference to planktonic bacteria and rather less attention has been directed towards bacteria in the biofilm mode of growth. In this study, the activity of a kilohertz-driven atmospheric pressure non-thermal plasma jet, operated in a helium oxygen mixture, against Pseudomonas aeruginosa in vitro biofilms was evaluated. Pseudomonas aeruginosa biofilms exhibit marked susceptibility to exposure of the plasma jet effluent, following even relatively short (∼10′s s) exposure times. Manipulation of plasma operating conditions, for example, plasma operating frequency, had a significant effect on the bacterial inactivation rate. Survival curves exhibit a rapid decline in the number of surviving cells in the first 60 seconds followed by slower rate of cell number reduction. Excellent anti-biofilm activity of the plasma jet was also demonstrated by both confocal scanning laser microscopy and metabolism of the tetrazolium salt, XTT, a measure of bactericidal activity.
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影响因子：
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