Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A.
Rotavirus A Inoculation and Oral Vitamin A Supplementation of Vitamin A Deficient Pregnant Sows Enhances Maternal Adaptive Immunity and Passive Protection of Piglets against Virulent Rotavirus A.
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DOI：
10.3390/v14112354
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发表时间：
2022-10-26
期刊：
影响因子：
--
通讯作者：
Saif LJ
中科院分区：
文献类型：
--
作者：
Chepngeno J;Amimo JO;Michael H;Jung K;Raev S;Lee MV;Damtie D;Mainga AO;Vlasova AN;Saif LJ
本研究的目的是确定维生素 A 缺乏 (VAD)/补充 (±VA) 和 A 组 RV (RVA) 母猪免疫对 RVA 血清阳性经产母猪的免疫反应（记忆反应）以及对仔猪被动保护免受 RVA 攻击的影响。我们的结果表明，VAD 模拟母猪在仔猪 RVA 攻击后 1-5 天增加了 RVA RNA 脱落，并且在整个实验过程中，它们的窝仔猪 RVA 脱落和腹泻频率增加。 VAD 降低了记忆 B 细胞频率，而补充 VA 则增加了接种 RVA 的 VAD 母猪的血液、乳汁和组织中 RVA 特异性 IgA/IgG 抗体 (Ab) 分泌细胞 (ASC) 的数量。补充 VA 的 VAD 母猪的血液、乳汁/初乳、肠内容物和组织中 RVA 特异性 IgA/IgG ASC 数量增加，表明 VA 在 B 细胞免疫和运输到组织中的作用。我们还观察到，接种 RVA 的母猪血清和牛奶中病毒中和抗体滴度最高，而 VAD 母猪补充 VA 和接种 RVA 增加了母猪初乳中 IgA+ B 细胞的频率。总之，我们证明，对接种 RVA 的 VAD 母猪每日口服 VA 补充剂（妊娠中期至整个哺乳期）可改善其肠道 - 乳腺 - IgA 免疫轴的功能，减少病毒 RNA 脱落、腹泻并增加哺乳仔猪的体重增加。
The aim of this study was to determine the impact of vitamin A deficiency (VAD)/supplementation (±VA) and group A RV (RVA) maternal immunization of RVA seropositive multiparous pregnant sows, on their immune responses (anamnestic response) and on passive protection of their piglets against RVA challenge. Our results showed that VAD- mock sows had increased RVA RNA shedding at 1–5 days post piglet RVA challenge, and their litters had increased RVA shedding and diarrhea frequency throughout the experiment. VAD decreased memory B cell frequencies while VA supplementation increased RVA specific IgA/IgG antibody (Ab) secreting cell (ASC) numbers in blood, milk, and tissues of RVA inoculated VAD sows. The increased numbers of RVA specific IgA/IgG ASCs in blood, milk/colostrum, intestinal contents, and tissues in VA supplemented VAD sows, suggest a role of VA in B cell immunity and trafficking to tissues. We also observed that RVA inoculated sows had the highest viral neutralizing Ab titers in serum and milk while VA supplementation of VAD sows and RVA inoculation increased IgA+ B cell frequencies in sow colostrum. In summary, we demonstrated that daily oral VA-supplementation (2nd trimester-throughout lactation) to RVA inoculated VAD sows improved the function of their gut-mammary-IgA immunological axis, reducing viral RNA shedding, diarrhea, and increasing weight gain in suckling piglets.
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影响因子：
4.8
作者：
Kaufman, Yardana;Ma, Li;Washington, Ilyas
通讯作者：
Washington, Ilyas
影响因子：
6.4
作者：
DeCicco, KL;Zolfaghari, R;Ross, AC
通讯作者：
Ross, AC
影响因子：
7
作者：
DeCicco, KL;Ross, AC
通讯作者：
Ross, AC
影响因子：
5.9
作者：
de Medeiros PHQS;Pinto DV;de Almeida JZ;Rêgo JMC;Rodrigues FAP;Lima AÂM;Bolick DT;Guerrant RL;Oriá RB
通讯作者：
Oriá RB
影响因子：
3.7
作者：
Chattha KS;Kandasamy S;Vlasova AN;Saif LJ
通讯作者：
Saif LJ