Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.
Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.
复制标题
DOI：
10.1007/s00223-016-0197-4
复制
发表时间：
2017-01
影响因子：
4.2
通讯作者：
Lytton, J.
中科院分区：
文献类型：
--
作者：
Bronckers, A. L. J. J.;Jalali, R.;Lytton, J.
形成的釉质暴露在氟化物中会导致低矿化牙釉质的形成。我们检测了成釉细胞中NCKX4/SLC24A4钙离子转运体的低表达是否导致了釉质的矿化。在野生型和NCKX4缺失型小鼠的釉质器官上检测了三种商品化的抗NCKX4抗体，其中一种是特异性的。这种抗体在成熟的成釉细胞的顶端质膜上有明显的染色，开始于成釉细胞的早期。免疫阳性的成釉细胞层有狭窄的间隙，未见免疫染色或染色减弱。在氟中毒的小鼠切牙中，用免疫印迹法检测成釉细胞中NCKX4蛋白的量与非氟中毒的成釉细胞中的NCKX4蛋白的数量没有差异。然而，氟中毒成釉细胞顶端质膜的免疫染色明显减少或缺失，这表明NCKX4向顶膜的转运明显减少。氟暴露可能减少成熟期成釉细胞NCKX4介导的钙转运，从而延迟成釉细胞的调控，减少釉质矿化。
Exposure of forming enamel to fluoride results into formation of hypomineralized enamel. We tested whether enamel hypomineralization was caused by lower expression of the NCKX4/SLC24A4 Ca2+-transporter by ameloblasts. Three commercial antibodies against NCKX4 were tested on enamel organs of wild-type and Nckx4-null mice, one of which (a mouse monoclonal) was specific. This antibody gave a prominent staining of the apical plasma membranes of maturation ameloblasts, starting at early maturation. The layer of immuno-positive ameloblasts contained narrow gaps without immunostaining or with reduced staining. In fluorotic mouse incisors, the quantity of NCKX4 protein in ameloblasts as assessed by western blotting was not different from that in non-fluorotic ameloblasts. However, immunostaining of the apical plasma membranes of fluorotic ameloblasts was strongly reduced or absent suggesting that trafficking of NCKX4 to the apical membrane was strongly reduced. Exposure to fluoride may reduce NCKX4-mediated transport of Ca2+ by maturation stage ameloblasts which delays ameloblast modulation and reduces enamel mineralization.
登录
查看更多内容
影响因子：
7.6
作者：
Lyaruu, D. M.;Medina, J. F.;Bronckers, A. L. J. J.
通讯作者：
Bronckers, A. L. J. J.
影响因子：
5.6
作者：
Lacruz, Rodrigo S.;Smith, Charles E.;Moffatt, Pierre;Chang, Eugene H.;Bromage, Timothy G.;Bringas, Pablo, Jr.;Nanci, Antonio;Baniwal, Sanjeev K.;Zabner, Joseph;Welsh, Michael J.;Kurtz, Ira;Paine, Michael L.
通讯作者：
Paine, Michael L.
影响因子：
25
作者：
通讯作者：
--
影响因子：
2
作者：
Wang SK;Hu Y;Yang J;Smith CE;Nunez SM;Richardson AS;Pal S;Samann AC;Hu JC;Simmer JP
通讯作者：
Simmer JP
影响因子：
4.8
作者：
Li, XF;Kraev, AS;Lytton, J
通讯作者：
Lytton, J