Single-cell RNA sequencing reveals heterogeneity and differential expression of decidual tissues during the peripartum period.
Single-cell RNA sequencing reveals heterogeneity and differential expression of decidual tissues during the peripartum period.
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单细胞RNA测序揭示围产期蜕膜组织的异质性和差异表达
DOI：
10.1111/cpr.12967
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发表时间：
2021-03
影响因子：
8.5
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Huang J;Li Q;Peng Q;Xie Y;Wang W;Pei C;Zhao Y;Liu R;Huang L;Li T;Xie L;Zhang J;Dai L;Chen J;Sun J;Zhang W
蜕膜是一种接触母体和胎儿的组织，由于它的位置，它对临产至关重要。由于蜕膜组织的异质性，研究其在围产期的作用具有挑战性。在这里，我们分析了单细胞分辨率的围产期蜕膜的转录。在分娩前后对29~231个蜕膜细胞进行单细胞RNA测序，以确定转录本的特征。8种主要细胞类型(包括内皮细胞、成纤维细胞)和蜕膜基质细胞、绒毛外滋养细胞和T细胞具有不同的功能。与分娩前比较，分娩后蜕膜基质细胞、绒毛外滋养细胞及T细胞亚群均有不同程度的活化。此外，激活涉及多种功能，如细胞增殖，以及几个途径，如激活蛋白1途径。假时序结果显示蜕膜基质细胞和绒毛外滋养层细胞亚型的分化，提示这些亚型在蜕膜(蜕膜基质细胞)和侵袭性(绒毛外滋养层细胞)方面的不均一性。围产期蜕膜组织是异质性的。这项研究揭示了单细胞分辨率下蜕膜及其成分的变化；这些发现为围产期蜕膜的研究提供了一个新的视角。采用单细胞RNA测序技术分析分娩前后蜕膜组织的转录本。鉴定出8种主要细胞类型和16个细胞簇，并发现它们具有不同的功能。分娩后观察到不同细胞亚型不同程度的激活。这些结果揭示了单细胞分辨率下围产期蜕膜细胞的异质性。
The decidua is a tissue that contacts both maternal and foetal components and is pivotal to labour onset due to its location. Due to the heterogeneity of decidual tissue, it is challenging to study its role in the peripartum period. Herein, we analysed the transcriptomes of peripartum decidua at single‐cell resolution. Single‐cell RNA sequencing was performed for 29 231 decidual cells before and after delivery to characterize the transcriptomes. Eight major cell types (including endothelial cells, fibroblasts) and subtypes of decidual stromal cells, extravillous trophoblasts and T cells were identified and found to have various functions. Compared with before delivery, the activation of decidual stromal cell, extravillous trophoblast and T‐cell subtypes to different degrees was observed after delivery. Furthermore, the activation involved multiple functions, such as cell proliferation, and several pathways, such as the activator protein 1 pathway. The results of pseudotemporal ordering showed differentiation of decidual stromal cell and extravillous trophoblast subtypes, suggesting inhomogeneity of these subgroups in decidualization (decidual stromal cell) and invasion (extravillous trophoblast). The peripartum decidual tissue is heterogeneous. This study revealed changes in the decidua and its components at single‐cell resolution; these findings provide a new perspective for the study of peripartum decidua. Single‐cell RNA sequencing was used to characterize the transcriptomes of decidua before and after delivery. Eight major cell types and sixteen clusters were identified and found to have various functions. The activation of various cell subtypes to different degrees was observed after delivery. These reveal the heterogeneity of peripartum decidual cells at single‐cell resolution
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影响因子：
16.6
作者：
MacParland SA;Liu JC;Ma XZ;Innes BT;Bartczak AM;Gage BK;Manuel J;Khuu N;Echeverri J;Linares I;Gupta R;Cheng ML;Liu LY;Camat D;Chung SW;Seliga RK;Shao Z;Lee E;Ogawa S;Ogawa M;Wilson MD;Fish JE;Selzner M;Ghanekar A;Grant D;Greig P;Sapisochin G;Selzner N;Winegarden N;Adeyi O;Keller G;Bader GD;McGilvray ID
通讯作者：
McGilvray ID
1996 - 2015年中国国家和地区全因原因和特定儿童死亡率：系统分析，对可持续发展目标有影响。
DOI：
10.1016/s2214-109x(16)30334-5
发表时间：
2017-02
期刊：
The Lancet. Global health
影响因子：
--
作者：
He C;Liu L;Chu Y;Perin J;Dai L;Li X;Miao L;Kang L;Li Q;Scherpbier R;Guo S;Rudan I;Song P;Chan KY;Guo Y;Black RE;Wang Y;Zhu J
通讯作者：
Zhu J
影响因子：
3.6
作者：
El-Azzamy, Haidy;Dambaeva, Svetlana V.;Kwak-Kim, Joanne
通讯作者：
Kwak-Kim, Joanne
Toll 样受体 4 在妊娠期间通过 NF-κB/P38MAPK 信号通路上调促炎细胞因子和 CAP 表达，有助于子宫激活
影响因子：
5.6
作者：
Chen, Zixi;Liu, Qiaoli;Kang, Xiangdong
通讯作者：
Kang, Xiangdong
影响因子：
3.8
作者：
Liu, X.;Gu, W.;Li, X.
通讯作者：
Li, X.