Polydatin Protects Bovine Mammary Epithelial Cells Against Zearalenone-Induced Apoptosis By Inhibiting Oxidative Responses and Endoplasmic Reticulum Stress.
Polydatin Protects Bovine Mammary Epithelial Cells Against Zearalenone-Induced Apoptosis By Inhibiting Oxidative Responses and Endoplasmic Reticulum Stress.
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虎杖苷通过抑制氧化反应和内质网应激保护牛乳腺上皮细胞免受玉米赤霉烯酮诱导的凋亡。
DOI：
10.3390/toxins13020121
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发表时间：
2021-02-05
期刊：
影响因子：
4.2
通讯作者：
Tian Y
中科院分区：
文献类型：
--
作者：
Fu Y;Jin Y;Shan A;Zhang J;Tang H;Shen J;Zhou C;Yu H;Fang H;Zhao Y;Wang J;Tian Y
玉米赤霉烯酮（ZEA）是镰刀菌属真菌毒素，可引起牛乳腺上皮细胞（MAC-T）的内质网（ER）应激和凋亡。虎杖苷（PD）是从虎杖中提取的一种糖苷，具有抗氧化作用。本研究旨在探讨PD是否能减轻ZEA对牛乳腺上皮细胞（MAC-T）的损伤。我们发现，随着ZEA浓度的增加（0、7.5、15、30、60、90、120和240 μM），细胞活力逐渐降低。5、10和20 μM单独PD处理不影响细胞活力。后续研究再应用30 μM ZEA和5 μM PD处理细胞;结果显示，与ZEA处理组相比，ZEA + PD处理组有效降低了细胞氧化损伤。qPCR分析表明，相对于对照，ZEA处理显著上调ER应激相关基因的表达。然而，加入PD显着下调ER应激相关基因的表达。细胞凋亡检测结果显示，与ZEA处理组相比，ZEA + PD处理组下调Bax基因表达，上调Bcl-2基因表达，降低细胞凋亡率和Caspase-3活性。总之，这些结果表明，PD通过抑制氧化损伤和ER应激减少ZEA诱导的细胞凋亡。
Zearalenone (ZEA) is a mycotoxin of the Fusarium genus that can cause endoplasmic reticulum (ER) stress and Apoptosis in bovine mammary epithelial cells (MAC-T). Polydatin (PD), a glycoside purified from Polygonum cuspidatum, has antioxidant properties. This study aimed to explore whether PD can alleviate ZEA-induced damage on bovine mammary epithelial cells (MAC-T). We found that incasing the concentration of ZEA (0, 7.5, 15, 30, 60, 90, 120, and 240 μM) gradually decreased the cell viability. PD treatment alone at 5, 10, and 20 μM did not affect cell viability. Follow-up studies then applied 30 μM of ZEA and 5 μM of PD to treat cells; the results showed that the ZEA + PD treatment group effectively reduced cell oxidative damage compared with the ZEA treatment group. The qPCR analysis showed that ZEA treatment significantly up-regulated the expression of ER stress-related genes, relative to the control. However, adding PD significantly down-regulated the expression of ER stress-related genes. The cell apoptosis detection results showed that, compared with the ZEA treatment group, the ZEA + PD treatment group down-regulated the Bax gene and up-regulated the Bcl-2 gene expressions, which reduced the cell apoptosis rate and Caspase-3 activity. Taken together, these results indicate that PD reduces ZEA-induced apoptosis by inhibiting oxidative damage and ER stress.
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影响因子：
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