Concurrent loss and proliferation of astrocytes following lateral fluid percussion brain injury in the adult rat
Concurrent loss and proliferation of astrocytes following lateral fluid percussion brain injury in the adult rat
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成年大鼠侧方液体撞击脑损伤后星形胶质细胞同时丢失和增殖
DOI：
--
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发表时间：
1999
影响因子：
4.2
通讯作者：
E. Barbarese
中科院分区：
文献类型：
--
作者：
Sandra J. Hill‐Felberg;Tracy K. McIntosh;D. Oliver;R. Raghupathi;E. Barbarese
对侧向液压冲击(FP)脑损伤后1个月的海马区星形胶质细胞数量进行了分析。23只大鼠接受中度颅脑损伤，7只接受麻醉和手术。分别于伤后3天、1周、2周、4周处死动物，取脑组织进行组织化学分析。金升华染色显示，损伤大鼠同侧海马区星形胶质细胞密度在伤后3天显著降低，至伤后1周降至非损伤组星形胶质细胞总数的%。损伤后1个月，海马区星形胶质细胞密度已恢复至未损伤组的85%。为了研究创伤后新生星形胶质细胞的形成，用增殖细胞核抗原和胶质纤维酸性蛋白抗体进行免疫组织化学染色。在FP脑损伤后3d，血脑屏障受损的区域，增殖细胞核抗原和GFAP的阳性免疫标记最为丰富，伤后1个月仍未检测到。这些结果表明，星形胶质细胞损伤后的增殖可能是由血管来源释放的有丝分裂原引起的，可能是为了补偿损伤后观察到的星形胶质细胞的部分损失。J.神经学决议57：271-279,1999。©1999 Wiley-Liss，Inc.
Astrocyte populations were analyzed over a period of 1 month in the hippocampus following lateral fluid percussion (FP) brain injury. Rats (n = 23) were subjected either to a brain injury of moderate severity, or to anesthesia and surgery without injury (n = 7). At 3 days, 1, 2, or 4 weeks postinjury, subgroups of animals were sacrificed and the brains removed and sectioned for histochemical analysis. The density of astrocytes, identified with gold sublimate staining, decreased significantly in the ipsilateral hippocampus of injured rats 3 days following injury, eventually falling to 64% of the total astrocyte population present in uninjured animals by 1 week postinjury. One month postinjury, the density of hippocampal astrocytes had returned to 85% of the total number of astrocytes observed in the hippocampus of uninjured animals. In order to characterize the post‐traumatic formation of new astrocytes, immunohistochemistry was performed using antibodies to proliferating cell nuclear antigen (PCNA) and to glial fibriallary acidic protein (GFAP). Positive immunolabeling for both PCNA and GFAP was most abundant at 3 days following FP brain injury in regions where the blood brain barrier was compromised, and was not detectable by 1 month postinjury. These results indicate that astrocyte proliferation after injury may be evoked by mitogens released from vascular sources, and may be an attempt to compensate for some of the astrocytic cell loss observed after injury. J. Neurosci. Res. 57:271–279, 1999. © 1999 Wiley‐Liss, Inc.
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影响因子：
4.2
作者：
DIETRICH, WD;ALONSO, O;HALLEY, M
通讯作者：
HALLEY, M
DOI：
--
发表时间：
1995
期刊：
The American journal of pathology
影响因子：
--
作者：
A. Rink;K. Fung;J. Trojanowski;V. Lee;E. Neugebauer;Tracy K. McIntosh
通讯作者：
A. Rink;K. Fung;J. Trojanowski;V. Lee;E. Neugebauer;Tracy K. McIntosh
影响因子：
56.9
作者：
GIULIAN, D;LACHMAN, LB
通讯作者：
LACHMAN, LB
对创伤性脑损伤的轴突反应：反应性轴突变化、传入神经阻滞和神经可塑性。
影响因子：
4.2
作者：
Povlishock,JT;Erb,DE;Astruc,J
通讯作者：
Astruc,J