Nischarin is differentially expressed in rat brain and regulates neuronal migration.
Nischarin is differentially expressed in rat brain and regulates neuronal migration.
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DOI：
10.1371/journal.pone.0054563
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Ding Y;Zhang R;Zhang K;Lv X;Chen Y;Li A;Wang L;Zhang X;Xia Q
Nischarin是一种已知通过调节Rho GT3家族信号传导抑制乳腺癌细胞运动的蛋白质。然而，人们对它在神经系统中的位置和功能知之甚少。本研究的目的是研究Nischarin在成年啮齿动物脑中的区域和细胞表达及其功能。通过实时荧光定量PCR、免疫印迹分析和免疫组化检测，我们发现Nischarin广泛分布于整个脑组织，在大脑皮层和海马中表达较高。双标记显示Nischarin在神经元中表达，主要定位于核周区和富含F-actin的突起。Nischarin在脑中的表达模式被认为与其功能密切相关。这被我们从细胞迁移试验中发现的Nischarin调节神经元迁移所证实。这些结果为Nischarin在大鼠脑内不同区域和细胞类型中的分布提供了初步的调查。这可能有助于阐明其生理作用，并评估其潜在的临床意义。
Nischarin is a protein known to inhibit breast cancer cell motility by regulating the signaling of the Rho GTPase family. However, little is known about its location and function in the nervous system. The aim of the present study was to investigate the regional and cellular expression and functions of Nischarin in the adult rodent brain. As assessed by real-time PCR, Western blot analysis and immunostaining, we found that Nischarin was widely distributed throughout the brain, with a higher expression in the cerebral cortex and hippocampus. Double-labeling showed that Nischarin was expressed in neurons and was mainly located in the perinuclear region and F-actin-rich protrusions. The expression pattern of Nischarin in the brain was thought to be closely associated with its function. This was verified by our findings from cell migration assays that Nischarin regulated neuronal migration. These results provide a preliminary survey of the distribution of Nischarin in different regions and cell types in the rat brain. This might help to elucidate its physiological roles, and to evaluate its potential clinical implications.
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影响因子：
6.7
作者：
Cayre M;Canoll P;Goldman JE
通讯作者：
Goldman JE
DOI：
10.1093/jnci/djr350
发表时间：
2011-10-01
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Baranwal, Somesh;Wang, Yanfang;Alahari, Suresh K.
通讯作者：
Alahari, Suresh K.
影响因子：
4.2
作者：
Li, Aiqing;Zhou, Tianhua;Si, Jianmin
通讯作者：
Si, Jianmin
影响因子：
3.1
作者：
Rathinam, Rajamani;Berrier, Allison;Alahari, Suresh K.
通讯作者：
Alahari, Suresh K.
影响因子：
5.3
作者：
Ding, Yuemin;Milosavljevic, Tanja;Alahari, Suresh K.
通讯作者：
Alahari, Suresh K.