Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice.
Accelerated molecular breeding of a novel P/TGMS line with broad-spectrum resistance to rice blast and bacterial blight in two-line hybrid rice.
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DOI：
10.1186/s12284-018-0203-8
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发表时间：
2018-02-17
期刊：
影响因子：
--
通讯作者：
Mou T
中科院分区：
文献类型：
--
作者：
Mi J;Yang D;Chen Y;Jiang J;Mou H;Huang J;Ouyang Y;Mou T
选育抗病两系杂交稻是中国商品稻生产稳产的有效途径。通过分别导入水稻抗稻瘟病和抗白叶枯病的抗病基因Pi2和Xa7，提高了广占63-4S的抗稻瘟病和抗白叶枯病能力。通过回交、基因聚合育种和分子标记辅助选择，育成了携带Pi2、Xa7和Tms5的光温敏核不育系华1228S。基于200,000个SNP标记的下一代测序，HU1228S覆盖了87.6%的重复基因组，以及VE6219和YR7029-39供体基因组的4.5%和7.9%。感染7株供试的水稻白叶枯病菌。水稻菌株花1228S对6个白叶枯病菌株表现出0级抗性。此外，花1228S对稻瘟病分离物表现广谱抗性，抗性频率高达90.91%。在自然条件下，在重病压力下表现出较高的叶瘟和颈瘟抗性。重要的是，花1228S表现出与广占63-4S相同的育性-不育转换模式。与对照品种丰两优4号相比，华两优2821和华两优284表现出较强的抗逆性和较高的产量。这些结果表明，通过分子标记辅助选择策略导入两个R基因，华两优28S具有较大的增产稳产潜力。本文的在线版本(10.1186/s12284-0180203-8)包含补充材料，可供授权用户使用。
Breeding two-line hybrid rice with disease resistance is an effective approach to stabilize rice yield in commercial rice production of China. We improved the blast and bacterial blight resistance of Guangzhan63-4S, an elite photoperiod- and thermo-sensitive male sterile (P/TGMS) line widely used in two-line hybrid rice, by introducing the R genes Pi2 and Xa7 conferring resistance to rice blast and bacterial blight, respectively. Through the backcrossing and gene pyramiding breeding coupled with molecular marker-assisted selection, a new P/TGMS line Hua1228S carrying Pi2, Xa7, and tms5 was developed. Based on 200,000 SNP markers by next-generation sequencing, Hua1228S covered 87.6% of the recurrent genome, as well as 4.5% of the donor genome from VE6219 and 7.9% from YR7029–39. When infected with seven tested Xanthomonas oryzae pv. oryzae strains, Hua1228S conferred high resistance (0 level) to six bacterial blight strains. Moreover, Hua1228S showed broad-spectrum resistance to rice blast isolates with a high resistance frequency of 90.91%. High levels of resistance to leaf blast and neck blast were observed under heavy disease pressure in natural field. Importantly, Hua1228S showed identical fertility-sterility alteration pattern to Guangzhan63-4S. Thus, two hybrid combinations Hua Liangyou 2821 and Hua Liangyou 284 derived from Hua1228S exhibited enhanced resistance and higher yield compared with the control variety Feng Liangyou 4. These results indicate that Hua1228S has tremendous potentiality to increase and stabilize the rice yield, through the introgression of two R genes by marker-assisted selection strategy. The online version of this article (10.1186/s12284-018-0203-8) contains supplementary material, which is available to authorized users.
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