Redox feedback regulation of ANAC089 signaling alters seed germination and stress response.
Redox feedback regulation of ANAC089 signaling alters seed germination and stress response.
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DOI：
10.1016/j.celrep.2021.109263
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发表时间：
2021-06-15
期刊：
影响因子：
8.8
通讯作者：
Lorenzo O
中科院分区：
文献类型：
--
作者：
Albertos P;Tatematsu K;Mateos I;Sánchez-Vicente I;Fernández-Arbaizar A;Nakabayashi K;Nambara E;Godoy M;Franco JM;Solano R;Gerna D;Roach T;Stöggl W;Kranner I;Perea-Resa C;Salinas J;Lorenzo O
植物激素脱落酸（阿坝）和气体递质一氧化氮（NO）之间的相互作用调节种子萌发和萌发后的幼苗生长。我们发现，GAP 1（在阿坝和cPTIO 1发芽）编码的转录因子ANAC089与一个关键的膜结合结构域和细胞核定位。ANAC089突变体缺乏膜系域显示不敏感阿坝，盐，渗透和冷应力，揭示了阻遏功能。全基因组转录谱分析和DNA结合特异性表明，ANAC089调节阿坝和氧化还原相关基因。ANAC089截短突变体表现出更高的NO和更低的ROS和阿坝内源性水平，以及改变的巯基和二硫键稳态。一致地，ANAC089向细胞核的易位是由用NO清除剂和氧化还原相关化合物处理后细胞氧化还原状态的变化指导的。我们的研究结果表明，ANAC089是一个主调节器调节氧化还原稳态和NO水平，能够抑制阿坝的合成和信号在拟南芥种子萌发和非生物胁迫。GAP 1编码转录因子ANAC089，在种子和胁迫中表达细胞氧化还原状态的变化诱导ANAC089易位到细胞核ANAC089特异性结合控制种子萌发和非生物胁迫的基因ANAC089调节NO水平和细胞氧化还原并抑制阿坝合成和信号传导Albertos et al.报道了ANAC089转录因子从内质网中的膜锚定结构域裂解，由细胞氧化还原改变控制。一旦进入细胞核，ANAC089诱导氧化还原稳态相关基因并抑制ABA响应基因，以增加种子萌发和非生物胁迫期间的NO水平和阿坝不敏感性。
The interplay between the phytohormone abscisic acid (ABA) and the gasotransmitter nitric oxide (NO) regulates seed germination and post-germinative seedling growth. We show that GAP1 (germination in ABA and cPTIO 1) encodes the transcription factor ANAC089 with a critical membrane-bound domain and extranuclear localization. ANAC089 mutants lacking the membrane-tethered domain display insensitivity to ABA, salt, and osmotic and cold stresses, revealing a repressor function. Whole-genome transcriptional profiling and DNA-binding specificity reveals that ANAC089 regulates ABA- and redox-related genes. ANAC089 truncated mutants exhibit higher NO and lower ROS and ABA endogenous levels, alongside an altered thiol and disulfide homeostasis. Consistently, translocation of ANAC089 to the nucleus is directed by changes in cellular redox status after treatments with NO scavengers and redox-related compounds. Our results reveal ANAC089 to be a master regulator modulating redox homeostasis and NO levels, able to repress ABA synthesis and signaling during Arabidopsis seed germination and abiotic stress. GAP1 encodes transcription factor ANAC089, expressed in seeds and stress Changes in cellular redox status induce translocation of ANAC089 to the nucleus ANAC089 binds specifically to genes controlling seed germination and abiotic stress ANAC089 regulates NO levels and cell redox and represses ABA synthesis and signaling Albertos et al. report the cleavage of ANAC089 transcription factor from the membrane-anchoring domain in the endoplasmic reticulum, controlled by cell redox alterations. Once in the nucleus, ANAC089 induces redox homeostasis-related genes and represses ABA-responsive genes to increase NO levels and ABA insensitivity during seed germination and abiotic stress.
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影响因子：
14.8
作者：
Berger, Michael F.;Bulyk, Martha L.
通讯作者：
Bulyk, Martha L.
影响因子：
46.9
作者：
Berger, Michael F.;Philippakis, Anthony A.;Bulyk, Martha L.
通讯作者：
Bulyk, Martha L.
DOI：
10.1007/978-1-61779-231-1_20
发表时间：
2011-01-01
期刊：
SEED DORMANCY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Bailly, Christophe;Kranner, Ilse
通讯作者：
Kranner, Ilse
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF