Biosensing with nanofluidic diodes.
Biosensing with nanofluidic diodes.
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DOI：
10.1021/ja901120f
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发表时间：
2009-06-17
影响因子：
15
通讯作者：
Siwy ZS
中科院分区：
文献类型：
--
作者：
Vlassiouk I;Kozel TR;Siwy ZS
最近报道的具有高度非线性电流-电压特性的纳米流体二极管为构建不同的生物传感器提供了独特的可能性。这些传感器是基于分析物结合引起的单个锥形纳米孔壁上表面电荷的局部变化。分析物结合可以被检测为单个纳孔的离子电流整流的变化，其定义为单极性电压的电流与相反极性电压的电流的比率。在本文中，我们通过监测作为生物传感平台的纳米流控二极管整流度的变化，对各种生物传感途径进行了建模和实验研究。提出了一种炭疽芽孢杆菌胶囊聚γ-D-谷氨酸(γ-D-Glu)传感器的样机。用γDPGA的单抗对用于传感的纳米孔进行局部修饰。通过制备亲和素和链霉亲和素传感器，进一步证明了基于纠正度的传感原理。我们的设备还可以确定固定在表面的微量蛋白质的等电点。
Recently reported nanofluidic diodes with highly nonlinear current-voltage characteristics offer a unique possibility to construct different biosensors. These sensors are based on local changes of the surface charge on walls of single conical nanopores induced by binding of an analyte. The analyte binding can be detected as a change of the ion current rectification of single nanopores defined as a ratio of currents for voltages of one polarity, and currents for voltages of the opposite polarity. In this Article we provided both modeling and experimental studies of various biosensing routes based on monitoring changes of the rectification degree in nanofluidic diodes used as a biosensing platform. A prototype of a sensor for the capsular poly γ-D-glutamic acid (γDPGA) from Bacillus anthracis is presented. The nanopore used for the sensing was locally modified with the monoclonal antibody for γDPGA. The proof of principle of the rectification degree based sensing was further shown by preparation of sensors for avidin and streptavidin. Our devices also allowed for determination of isoelectric point of the minute amounts of proteins immobilized on the surface.
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DOI：
10.1016/s0168-583x(01)00722-4
发表时间：
2001-11-01
影响因子：
1.3
作者：
Apel, PY;Korchev, YE;Yoshida, M
通讯作者：
Yoshida, M
影响因子：
1.6
作者：
DEBLOIS, RW;BEAN, CP
通讯作者：
BEAN, CP
DOI：
10.1073/pnas.0401351101
发表时间：
2004-04-06
影响因子：
11.1
作者：
Kozel, TR;Murphy, WJ;Lyons, CR
通讯作者：
Lyons, CR
影响因子：
10.8
作者：
Karnik, Rohit;Duan, Chuanhua;Majumdar, Arun
通讯作者：
Majumdar, Arun
影响因子：
3.5
作者：
Ramirez, Patricio;Apel, Pavel Yu;Mafe, Salvador
通讯作者：
Mafe, Salvador