Photoacoustic imaging provides an in vivo assessment of the preeclamptic placenta remodeling and function in response to therapy.
Photoacoustic imaging provides an in vivo assessment of the preeclamptic placenta remodeling and function in response to therapy.
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DOI：
10.1016/j.placenta.2022.06.006
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发表时间：
2022-08
期刊：
影响因子：
3.8
通讯作者：
Bayer, Carolyn L.
中科院分区：
文献类型：
--
作者：
Lawrence, Dylan J.;Bayer, Carolyn L.
对于先兆子痫缺乏有效的治疗干预。该疾病的病因学中的一个中心因素是由于异常血管重塑导致的胎盘缺氧的发展。然而，目前缺乏评估潜在疗法对胎盘生长和重塑的影响的方法。在这里，我们开发和验证超声引导的光声成像方法来监测胎盘对治疗干预的反应。建立非侵入性工具来成像胎盘功能开辟了以前无法实现的胎盘治疗反应的理解。在先兆子痫的子宫灌注压降低（RUPP）大鼠模型中进行研究。临床前研究已经确定tempol，一种超氧化物歧化酶模拟物，和磷酸二酯酶抑制剂西地那非作为先兆子痫的潜在治疗药物，因为两者都改善了体内母体结局。在接受tempol（n = 8）或西地那非（n = 8）的RUPP大鼠中获得胎盘环境的PA图像，以评估治疗对胎盘氧合和血管重塑的纵向影响。用离体组织学分析验证成像测量。光谱光声成像非侵入性测量胎盘缺氧和受损的血管生长后两天的RUPP程序实施。西地那非显著改善（p < 0.05）RUPP动物的胎盘氧合并促进血管重塑，而用tempol治疗的RUPP动物具有减弱的胎盘治疗反应。我们证明，光声成像提供了胎盘氧合和血管重塑，以前无法获得的评估先兆子痫的治疗反应在体内的措施。这些成像工具具有巨大的潜力，可以加速寻找有效的先兆子痫治疗方法。
There is a lack of effective therapeutic interventions for preeclampsia. A central factor in the etiology of the disease is the development of placental hypoxia due to abnormal vascular remodeling. However, methods to assess the impact of potential therapies on placental growth and remodeling are currently lacking. Here, we develop and validate ultrasound-guided photoacoustic imaging methods to monitor the placental response to therapeutic intervention. Establishing non-invasive tools to image placental function opens up previously unachievable understandings of placental therapeutic response. Studies were performed in the reduced uterine perfusion pressure (RUPP) rat model of preeclampsia. Preclinical research has identified tempol, a superoxide dismutase mimetic, and the phosphodiesterase inhibitor sildenafil as potential therapeutics for preeclampsia, as both improve in vivo maternal outcomes. PA images of the placental environment were acquired in RUPP rats receiving tempol (n = 8) or sildenafil (n = 8) to assess the longitudinal effects of treatment on placental oxygenation and vascular remodeling. Imaging measurements were validated with ex vivo histological analysis. Spectral photoacoustic imaging non-invasively measured placental hypoxia and impaired vascular growth two days after the RUPP procedure was implemented. Sildenafil significantly improved (p < 0.05) placental oxygenation and promoted vascular remodeling in RUPP animals, while RUPP animals treated with tempol had a diminished placental therapeutic response. We demonstrate that photoacoustic imaging provides in vivo measures of placental oxygenation and vascular remodeling, a previously unobtainable assessment of preeclamptic therapeutic response. These imaging tools have tremendous potential to accelerate the search for effective therapies for preeclampsia.
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影响因子：
3.4
作者：
Alexander-Sefre, F;Singh, N;Jacobs, IJ
通讯作者：
Jacobs, IJ
影响因子：
7.2
作者：
通讯作者：
--
影响因子：
8.3
作者：
Alexander, BT
通讯作者：
Alexander, BT
DOI：
10.1152/ajpregu.00216.2013
发表时间：
2013-08-01
影响因子：
2.8
作者：
George, Eric M.;Palei, Ana C.;Granger, Joey P.
通讯作者：
Granger, Joey P.
影响因子：
4.6
作者：
Lawrence, Dylan J.;Escott, Megan E.;Bayer, Carolyn L.
通讯作者：
Bayer, Carolyn L.