Out-of-equilibrium microcompartments for the bottom-up integration of metabolic functions.
Out-of-equilibrium microcompartments for the bottom-up integration of metabolic functions.
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DOI：
10.1038/s41467-018-04825-1
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发表时间：
2018-06-19
影响因子：
16.6
通讯作者：
Baret JC
中科院分区：
文献类型：
--
作者：
Beneyton T;Krafft D;Bednarz C;Kleineberg C;Woelfer C;Ivanov I;Vidaković-Koch T;Sundmacher K;Baret JC
微区室中自我维持的代谢途径是生命系统的基石。从技术角度来看，此类途径是可靠设计非平衡功能的人造细胞的强制性先决条件。在这里，我们开发了一个微流体平台，用于微型化和分析由油包水液滴形成的人造微室中的代谢途径。在模块化方法中，我们在微室中集成了烟酰胺腺嘌呤二核苷酸 (NAD) 依赖性酶反应和 NAD 再生模块作为最小代谢。我们表明，微区室维持代谢活跃状态，直到底物完全消耗。可逆地，外部添加底物将微区室的代谢活动重新启动至活跃状态。因此，我们控制了数千个独立的单分散微区室的代谢状态，这是与构建大量代谢活性人工细胞相关的一步。生成能够进行非平衡代谢活动的人造细胞是合成生物学当前的挑战。在这里，作者使用微流体平台来集成和分析由油包水液滴形成的人造微区室中的最小代谢反应。
Self-sustained metabolic pathways in microcompartments are the corner-stone for living systems. From a technological viewpoint, such pathways are a mandatory prerequisite for the reliable design of artificial cells functioning out-of-equilibrium. Here we develop a microfluidic platform for the miniaturization and analysis of metabolic pathways in man-made microcompartments formed of water-in-oil droplets. In a modular approach, we integrate in the microcompartments a nicotinamide adenine dinucleotide (NAD)-dependent enzymatic reaction and a NAD-regeneration module as a minimal metabolism. We show that the microcompartments sustain a metabolically active state until the substrate is fully consumed. Reversibly, the external addition of the substrate reboots the metabolic activity of the microcompartments back to an active state. We therefore control the metabolic state of thousands of independent monodisperse microcompartments, a step of relevance for the construction of large populations of metabolically active artificial cells. Generating artificial cells able to carry out metabolic activities out-of-equilibrium is a current challenge in synthetic biology. Here the authors use a microfluidic platform for integration and analysis of minimal metabolic reactions in artificial microcompartments formed of water-in-oil droplets.
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影响因子：
15
作者：
HAMZA, MA;SERRATRICE, G;DELPUECH, JJ
通讯作者：
DELPUECH, JJ
影响因子：
46.9
作者：
Eyer, Klaus;Doineau, Raphael C. L.;Baudry, Jean
通讯作者：
Baudry, Jean
影响因子：
15
作者：
Deng, Nan-Nan;Yelleswarapu, Maaruthy;Huck, Wilhelm T. S.
通讯作者：
Huck, Wilhelm T. S.
影响因子：
64.8
作者：
Jeong, H;Tombor, B;Barabási, AL
通讯作者：
Barabási, AL
影响因子：
--
作者：
Baret, Jean-Christophe;Beck, Yannick;Griffiths, Andrew D.
通讯作者：
Griffiths, Andrew D.