Expression and inhibition of ADAMDEC1 in craniopharyngioma cells
Expression and inhibition of ADAMDEC1 in craniopharyngioma cells
复制标题
ADAMDEC1在颅咽管瘤细胞中的表达及抑制
DOI：
10.1179/1743132812y.0000000067
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发表时间：
2012-07
影响因子：
1.9
通讯作者：
Li, Qiang
中科院分区：
文献类型：
--
作者：
Liu, Liang;Zheng, Xiaomei;You, Chao;Li, Qiang
摘要背景和目的：颅咽管瘤是一种常见的颅内肿瘤，其复发率高，预后差。ADAM样decysin 1（ADAMDEC 1）是与肿瘤进展和侵袭行为相关的解整合素和金属蛋白酶（ADAM）家族的成员。本研究旨在检测和抑制ADAMDEC 1的表达，以了解是否可以抑制颅咽管瘤细胞的生长。方法：采用Western Blot和逆转录-聚合酶链反应（RT-PCR）检测ADAMDEC 1的表达。颅咽管瘤细胞，这是从手术切除后的肿瘤样本，培养或不与他莫昔芬。MTT法检测肿瘤细胞生长情况。结果：ADAMDEC 1 mRNA在颅咽管瘤细胞中有表达，而在正常脑组织中无表达。三苯氧胺不仅降低ADAMDEC 1 mRNA和蛋白表达，而且抑制颅咽管瘤细胞增殖。结论：ADAMDEC 1可能作为颅咽管瘤的一个新的标志物，他莫昔芬可以抑制颅咽管瘤细胞的生长和ADAMDEC 1的表达。
Abstract Background and purpose: Craniopharyngioma is a common intracranial tumor characterized by high recurrence rate and poor prognosis in spite of multidisciplinary approaches. The ADAM-like decysin 1 (ADAMDEC1) is a member of a disintegrin and metalloprotease (ADAM) family which correlates with tumor progression and aggressive behavior. This study aimed to detect and inhibit expression of ADAMDEC1 in order to see whether craniopharyngioma cell growth could be suppressed. Methods: ADAMDEC1 expression was detected by Western Blot analysis and reverse transcription-polymerase chain reaction (RT-PCR). Craniopharyngioma cells, which were obtained from tumor samples after surgical removal, were cultured with or without tamoxifen. MTT assay was used to examine tumor cell growth. Results: ADAMDEC1 mRNA was expressed in craniopharyngioma cell cultures, but it was not shown in normal brain tissue. Tamoxifen not only reduced ADAMDEC1 mRNA and protein expression, but also inhibited craniopharyngioma cell proliferation. Conclusions: ADAMDEC1 may serve as a novel marker for craniopharyngiomas, and tamoxifen could inhibit craniopharyngioma cell growth and ADAMDEC1 expression.
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影响因子：
2
作者：
T. Rossi;V. Iannuccelli;G. Coppi;E. Bruni;G. Baggio
通讯作者：
T. Rossi;V. Iannuccelli;G. Coppi;E. Bruni;G. Baggio
影响因子：
3.9
作者：
Jianguo Xu;Si-zhong Zhang;C. You;Si-qing Huang;Bo-wen Cai;Xiaojie Wang
通讯作者：
Jianguo Xu;Si-zhong Zhang;C. You;Si-qing Huang;Bo-wen Cai;Xiaojie Wang
影响因子：
10.6
作者：
Edwards DR;Handsley MM;Pennington CJ
通讯作者：
Pennington CJ
影响因子：
4.1
作者：
Jianguo Xu;C. You;Sizhong Zhang;Si-qing Huang;Bo-wen Cai;Zhenggui Wu;Hao Li
通讯作者：
Jianguo Xu;C. You;Sizhong Zhang;Si-qing Huang;Bo-wen Cai;Zhenggui Wu;Hao Li
DOI：
--
发表时间：
2005
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
J. Åhlén;J. Wejde;O. Brosjö;A. von Rosen;W. Weng;L. Girnita;O. Larsson;C. Larsson
通讯作者：
J. Åhlén;J. Wejde;O. Brosjö;A. von Rosen;W. Weng;L. Girnita;O. Larsson;C. Larsson