Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat.
Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat.
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DOI：
10.1093/jxb/erv006
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发表时间：
2015-04
影响因子：
6.9
通讯作者：
Wells DM
中科院分区：
文献类型：
--
作者：
Atkinson JA;Wingen LU;Griffiths M;Pound MP;Gaju O;Foulkes MJ;Le Gouis J;Griffiths S;Bennett MJ;King J;Wells DM
一个表型管道被用来量化小麦双单倍体作图群体中的幼苗根构型性状。QTL分析揭示了一个潜在的主效基因调节幼苗根系活力/生长。小麦（Triticum aestivum L.）已经显示出对于有效的建立是重要的，并且与成熟的植物性状如高度和产量相关。一个根表型管道，包括发芽纸为基础的屏幕结合图像分割和分析软件，开发和用于表征幼苗性状的94个双单倍体后代来自冬小麦品种Rialto和萨凡纳之间的杂交。在3个试验点进行了2年的田间试验，测定了成熟株高、籽粒产量和氮素吸收量。共检测到29个苗期根系性状的QTL。籽粒产量和氮素吸收的两个QTL分别与根系QTL共定位在2B和7 D染色体上。在所鉴定的29个根系QTL中，发现11个共定位于6D，其中4个获得了极显著的比值分数对数（>20）。这些结果表明，在染色体6D上存在调节幼苗根活力/生长的主效基因。
A phenotyping pipeline was used to quantify seedling root architectural traits in a wheat double haploid mapping population. QTL analyses revealed a potential major effect gene regulating seedling root vigour/growth. Seedling root traits of wheat (Triticum aestivum L.) have been shown to be important for efficient establishment and linked to mature plant traits such as height and yield. A root phenotyping pipeline, consisting of a germination paper-based screen combined with image segmentation and analysis software, was developed and used to characterize seedling traits in 94 doubled haploid progeny derived from a cross between the winter wheat cultivars Rialto and Savannah. Field experiments were conducted to measure mature plant height, grain yield, and nitrogen (N) uptake in three sites over 2 years. In total, 29 quantitative trait loci (QTLs) for seedling root traits were identified. Two QTLs for grain yield and N uptake co-localize with root QTLs on chromosomes 2B and 7D, respectively. Of the 29 root QTLs identified, 11 were found to co-localize on 6D, with four of these achieving highly significant logarithm of odds scores (>20). These results suggest the presence of a major-effect gene regulating seedling root vigour/growth on chromosome 6D.
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影响因子：
4.2
作者：
Waines JG;Ehdaie B
通讯作者：
Ehdaie B
影响因子：
5.1
作者：
Tuberosa, R;Sanguineti, MC;Conti, S
通讯作者：
Conti, S
影响因子：
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作者：
Bonser, AM;Lynch, J;Snapp, S
通讯作者：
Snapp, S
影响因子：
5.8
作者：
BLUM, A;ARKIN, GF
通讯作者：
ARKIN, GF
影响因子：
4.9
作者：
Liao, H;Rubio, G;Lynch, JP
通讯作者：
Lynch, JP