Frenolicins C-G, pyranonaphthoquinones from Streptomyces sp. RM-4-15.
Frenolicins C-G, pyranonaphthoquinones from Streptomyces sp. RM-4-15.
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DOI：
10.1021/np400231r
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发表时间：
2013-08-23
影响因子：
5.1
通讯作者：
Thorson JS
中科院分区：
文献类型：
--
作者：
Wang X;Shaaban KA;Elshahawi SI;Ponomareva LV;Sunkara M;Zhang Y;Copley GC;Hower JC;Morris AJ;Kharel MK;Thorson JS
选择阿巴拉契亚活跃的煤火地点用于分离属于放线菌纲的细菌菌株。高分辨率电喷雾电离质谱（HR-ESI-MS）和紫外（UV）吸收曲线从分离提取物的天然产物数据库的比较表明链霉菌属RM-4-15产生独特的代谢产物。从该菌株的发酵液中分离得到4个新的吡喃萘醌类化合物：frenolicins C-F（1-4），沿着3个已知的类似物：frenolicin（6），frenolicin B（7）和UCF 76-A（8）。从提供有18 mg/L氯化钪的发酵中分离出另外的新类似物弗伦诺利菌素G（5）沿着的两种已知化合物脱氧弗伦诺利菌素（9）和UCF 13（10）--据我们所知，这是第一个实施例，其中氯化钪补充导致确认产生新的细菌次级代谢物。根据光谱分析和化学修饰鉴定了结构1-5。虽然弗氏菌素最为人所知的是它们的抗球虫活性，但本研究揭示化合物6-9对人肺癌细胞系（A549）表现出中等的细胞毒性，从而延长了这种特权支架的抗癌SAR。
Appalachian active coal fire sites were selected for the isolation of bacterial strains belonging to the class actinobacteria. A comparison of high resolution electrospray ionization mass spectrometry (HR-ESI-MS) and ultraviolet (UV) absorption profiles from isolate extracts to natural product databases suggested Streptomyces sp. RM-4-15 to produce unique metabolites. Four new pyranonaphthoquinones, frenolicins C–F (1–4), along with three known analogues, frenolicin (6), frenolicin B (7), and UCF76-A (8), were isolated from the fermentation of this strain. An additional new analogue frenolicin G (5) along with two known compounds, deoxyfrenolicin (9) and UCF 13 (10), were isolated from the fermentation supplied with 18 mg/L of scandium chloride - the first example, to the best of our knowledge, wherein scandium chloride supplementation led to the confirmed production of new bacterial secondary metabolites. Structures 1–5 were elucidated on the basis of spectral analysis and chemical modification. While frenolicins are best known for their anticoccidial activity, the current study revealed compounds 6–9 to exhibit moderate cytotoxicity against the human lung carcinoma cell line (A549) and thereby extends the anticancer SAR for this privileged scaffold.
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唤醒沉默的生物合成基因簇以及将孤儿簇与微生物中的天然产物联系起来的最新进展。
影响因子：
7.8
作者：
Chiang, Yi-Ming;Chang, Shu-Lin;Oakley, Berl R.;Wang, Clay C. C.
通讯作者：
Wang, Clay C. C.
影响因子：
5
作者：
Ochi, Kozo;Hosaka, Takeshi
通讯作者：
Hosaka, Takeshi
影响因子：
3.3
作者：
IWAI, Y;KORA, A;OMURA, S
通讯作者：
OMURA, S
影响因子：
5.1
作者：
Abdelfattah, Mohamed S.;Kharel, Madan Kumar;Hitron, John Andrew;Baig, Irfan;Rohr, Juergen
通讯作者：
Rohr, Juergen
DOI：
10.1007/bf02908688
发表时间：
1984-01-01
期刊：
CARLSBERG RESEARCH COMMUNICATIONS
影响因子：
--
作者：
MARFEY, P
通讯作者：
MARFEY, P