Extract of Scutellaria baicalensis inhibits dengue virus replication.
Extract of Scutellaria baicalensis inhibits dengue virus replication.
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DOI：
10.1186/1472-6882-13-91
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发表时间：
2013-04-29
影响因子：
--
通讯作者：
Abubakar S
中科院分区：
文献类型：
--
作者：
Zandi K;Lim TH;Rahim NA;Shu MH;Teoh BT;Sam SS;Danlami MB;Tan KK;Abubakar S
黄芩（S.黄芩）是一种传统的中草药，已被证明具有许多健康益处。本研究以体外抗病毒活性为指标，评价了丹参水提物的抗病毒活性。黄芩对所有四种登革病毒（DENV）血清型的抗性。S.采用微波能量蒸汽蒸发法（MEGHE™）制备黄芩苷，并在Vero细胞中采用焦点形成单位还原试验（FFURA）评价黄芩苷的抗登革病毒复制活性。使用定量实时聚合酶链反应（qRT-PCR）测定来确定实际登革病毒RNA拷贝数。黄芩素的存在，已知抑制登革病毒复制的类黄酮，是通过质谱测定的。S.对于不同的DENV血清型，在DENV吸附后，黄芩提取物对Vero细胞的作用范围为86.59至95.19 μg/mL。当细胞在病毒吸附时用提取物处理不同DENV血清型时，IC 50值降至56.02至77.41 μg/mL。该提取物对细胞外感染性病毒颗粒显示出有效的直接杀病毒活性，对于所有DENV血清型的IC 50范围为74.33至95.83 μg/mL。当在病毒接种前2小时预处理细胞时，观察到较弱的预防作用，IC 50值范围为269.9 - 369.8 μg/mL。黄芩素在S.黄芩提取物为~1%（1.03 μg/gm干提取物）。我们的研究证实了S. baicalensis对所有四种研究的DENV血清型。提取物降低了DENV在Vero细胞中的感染性和复制。该提取物富含黄芩素，可以考虑用于抗DENV治疗剂的潜在开发。
Scutellaria baicalensis (S. baicalensis) is one of the traditional Chinese medicinal herbs that have been shown to possess many health benefits. In the present study, we evaluated the in vitro antiviral activity of aqueous extract of the roots of S. baicalensis against all the four dengue virus (DENV) serotypes. Aqueous extract of S. baicalensis was prepared by microwave energy steam evaporation method (MEGHE™), and the anti-dengue virus replication activity was evaluated using the foci forming unit reduction assay (FFURA) in Vero cells. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to determine the actual dengue virus RNA copy number. The presence of baicalein, a flavonoid known to inhibit dengue virus replication was determined by mass spectrometry. The IC50 values for the S. baicalensis extract on Vero cells following DENV adsorption ranged from 86.59 to 95.19 μg/mL for the different DENV serotypes. The IC50 values decreased to 56.02 to 77.41 μg/mL when cells were treated with the extract at the time of virus adsorption for the different DENV serotypes. The extract showed potent direct virucidal activity against extracellular infectious virus particles with IC50 that ranged from 74.33 to 95.83 μg/mL for all DENV serotypes. Weak prophylactic effects with IC50 values that ranged from 269.9 to 369.8 μg/mL were noticed when the cells were pre-treated 2 hours prior to virus inoculation. The concentration of baicalein in the S. baicalensis extract was ~1% (1.03 μg/gm dried extract). Our study demonstrates the in vitro anti-dengue virus replication property of S. baicalensis against all the four DENV serotypes investigated. The extract reduced DENV infectivity and replication in Vero cells. The extract was rich in baicalein, and could be considered for potential development of anti-DENV therapeutics.
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